Md 4017

Extractions were performed using a sonicator (VWR Ultrasound cleaner, model-USC 1700D). Analytical TLC was carried out with silica gel 60 F₂₅₄ precoated aluminum sheets (Merck Art. 1.05554). Compounds on TLC were located using a UV lamp and by heating after spraying with acidic anisaldehyde. Silica gel (Fluka 60741, Merck Art. 7734 and 9385) and Sephadex LH-20 were used for column chromatography. Preparative thin layer chromatography (PTLC) was carried out using silica gel 60 F₂₅₄ precoated glass plates (Merck Art 1.05715). ¹H NMR and ¹³C NMR were recorded on a Bruker DRX500 (500 MHz for ¹H and 125 MHz for ¹³C) or JEOL JMN-AL300 (300 MHz for ¹H and 75 MHz for ¹³C) spectrometer in CD₃OD or CDCl₃ solution at 20 °C. Optical rotations were measured on a JASCO P-2200 polarimeter. IR spectra were measured on a JASCO FT/IR-460 spectrometer. UV spectra were recorded on a JASCO MD-4017 photo diode array detector. Positive-ion FABMS were obtained on a JEOL JMX-AX505HA spectrometer.

HPAC was performed with a HSA-coated column (Faisal et al. 2018 (link)). The HPLC system (Jasco, Tokyo, Japan) used for the analysis included an autosampler (AS-4050), a binary pump (PU-4180), and a diode-array detector (MD-4017). A 5-μL volume of samples (ZAN: 200 μmol/L; ZEN, α-ZAL, β-ZAL, and Z14S: 100 μmol/L; Z14G: 50 μmol/L) was driven through a pre-column filter (Waters, Milford, MA, USA) linked to an immobilized HSA-coated HPAC column (Chiralpak^® HSA, 50 × 3.0 mm, 5 μm; Daicel, Tokyo, Japan). The isocratic elution was performed with 0.5-mL/min flow rate at room temperature. The mobile phase contained isopropanol (HPLC grade; VWR, Debrecen, Hungary) and 0.01 mol/L pH 7.0 ammonium acetate buffer (15:85 v/v%). Mycotoxins were detected at 235 nm, and chromatograms were evaluated with ChromNAV software.