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Unlabelled counting beads

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Unlabelled counting beads are small, spherical objects used for counting and quantifying purposes in various laboratory and educational settings. They are typically made of materials such as plastic or glass and are available in a range of colors. The core function of these beads is to provide a simple, versatile means of keeping track of numerical values or quantities.

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Lab products found in correlation

Cell dissociation buffer enzyme free pbs based, by Thermo Fisher Scientific (2 mentions) Facscanto 2, by BD (2 mentions)

2 protocols using unlabelled counting beads

1

Quantifying Neutrophil Adhesion to Inflamed Endothelium

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4×104 HUVEC were seeded in M199 20% FBS in a 96-multiwell plate, previously coated with 0.1% gelatin. After 12 hours, HUVEC were stimulated with 5 ng/ml IL-1α in DMEM 10% FBS at 37°C. After 4 hours the endothelial monolayer was thoroughly washed and 2.5×105 WBC isolated indistinctly from Tie2;Metwt/wt and Tie2;Metlox/lox mice or from WT→WT and KO→WT transplanted mice, were seeded on top of it, with or without murine HGF (50 ng/ml). After 15 minutes non-adherent cells were washed out whereas adherent cells were detached by using a Cell Dissociation Buffer Enzyme Free PBS-Based (Gibco). Cells were stained for Ly6G (clone 1A8), washed and resuspended in PBS-BSA 0.1% with unlabelled counting beads (BD Bioscience) and quantified by FACS Canto II (BD Bioscience).
Finisguerra V., Di Conza G., Di Matteo M., Serneels J., Costa S., Thompson A.A., Wauters E., Walmsley S., Prenen H., Granot Z., Casazza A, & Mazzone M. (2015). MET is required for the recruitment of anti-tumoural neutrophils. Nature, 522(7556), 349-353.
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2

Quantifying Neutrophil Adhesion to Inflamed Endothelium

Check if the same lab product or an alternative is used in the 5 most similar protocols
4×104 HUVEC were seeded in M199 20% FBS in a 96-multiwell plate, previously coated with 0.1% gelatin. After 12 hours, HUVEC were stimulated with 5 ng/ml IL-1α in DMEM 10% FBS at 37°C. After 4 hours the endothelial monolayer was thoroughly washed and 2.5×105 WBC isolated indistinctly from Tie2;Metwt/wt and Tie2;Metlox/lox mice or from WT→WT and KO→WT transplanted mice, were seeded on top of it, with or without murine HGF (50 ng/ml). After 15 minutes non-adherent cells were washed out whereas adherent cells were detached by using a Cell Dissociation Buffer Enzyme Free PBS-Based (Gibco). Cells were stained for Ly6G (clone 1A8), washed and resuspended in PBS-BSA 0.1% with unlabelled counting beads (BD Bioscience) and quantified by FACS Canto II (BD Bioscience).
Finisguerra V., Di Conza G., Di Matteo M., Serneels J., Costa S., Thompson A.A., Wauters E., Walmsley S., Prenen H., Granot Z., Casazza A, & Mazzone M. (2015). MET is required for the recruitment of anti-tumoural neutrophils. Nature, 522(7556), 349-353.
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