Western blots were performed as described [34 (link)]. Briefly, proteins were separated by gel electrophoresis, transferred to the Hybond-P PVDF membrane, blocked with 2% milk in PBST, incubated with primary and HRP secondary antibodies, treated with luminol substrate and developed on a film. Mouse monoclonal anti-caspase 8 (Sigma-Aldrich), mouse monoclonal anti-caspase 9 (Sigma-Aldrich), rabbit anti-caspase 3 (Sigma-Aldrich), rat monoclonal anti-caspase 12 (Sigma-Aldrich) and rabbit polyclonal antibody against Bim (amino acids 4–195 of BimEL form) were all used at a 1:1000 dilution for Western blots.
Mouse monoclonal anti caspase 8
Manufactured by Merck Group
Mouse monoclonal anti-caspase 8 is a laboratory reagent used to detect the presence and measure the levels of caspase 8 protein in biological samples. Caspase 8 is a key enzyme involved in the initiation of the apoptosis (programmed cell death) pathway. This product can be used in various immunodetection techniques, such as Western blotting, ELISA, and immunohistochemistry, to study the role of caspase 8 in cellular processes.
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Lab products found in correlation
Rabbit anti caspase 3, by Merck Group (2 mentions)
Mouse monoclonal anti caspase 9, by Merck Group (2 mentions)
Rat monoclonal anti caspase 12, by Merck Group (2 mentions)
Rabbit anti cox 4, by Cell Signaling Technology (1 mentions)
Rabbit anti bax, by Cell Signaling Technology (1 mentions)
Rabbit anti jnk, by Cell Signaling Technology (1 mentions)
Rabbit anti cytochrome c, by Cell Signaling Technology (1 mentions)
Rabbit anti apaf 1, by Cell Signaling Technology (1 mentions)
Rabbit anti bcl 2, by Cell Signaling Technology (1 mentions)
2 protocols using mouse monoclonal anti caspase 8
1
Western Blot Analysis of Apoptosis Markers
2
Western Blot Analysis of Apoptosis Markers
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Western blots were performed as described [38] (link). Mouse monoclonal anti-Caspase 8 (Sigma), mouse monoclonal anti-Caspase 9 (Sigma), rabbit anti-Caspase 3 (Sigma), rat monoclonal anti-Caspase 12 (Sigma), rabbit polyclonal antibody against Bim (amino acids 4–195 of BimEL form), rabbit anti-Cytochrome C (Cell Signaling), goat polyconal anti-HP1 (Santa Cruz), rabbit anti-CoxIV (Cell Signaling), rabbit anti-phospho-JNK (Thr183/Tyr185) (81E11) (Cell Signaling), rabbit anti-JNK (Cell Signaling), mouse monoclonal anti-CHOP (L63F7) (Cell Signaling), rabbit anti-Apaf-1 (Cell Signaling), rabbit anti-Bcl2 (Cell Signaling), rabbit polyclonal anti-ATF-6 (Santa Cruz), rabbit anti-Bax (Cell Signaling) were all used at a 1:1000 dilution for western blots.
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