Anti o linked n acetylglucosamine antibody rl2 ma1 072

The anti-O-linked N-acetylglucosamine antibody (RL2) (MA1–072) was purchased from ThermoFisher Scientific, and the anti-O-GlcNAcase (OGA) antibody produced in rabbit (SAB4200311) was purchased from Sigma Aldrich. The anti-OGT (AL-35, AL-28) and OGA (AL-345) antibodies were rabbit and chicken polyclonal antibodies, respectively, and were gracious gifts from the Laboratory of Gerald Hart in the Department of Biological Chemistry at the Johns Hopkins University School of Medicine. These antibodies have been used extensively to characterize the O-GlcNAc network in several model systems (Lanza et al. 2016 (link); Slawson et al. 2008 (link); Slawson et al. 2005 (link); Tan et al. 2013 (link); Wang et al. 2010 (link)). Rabbit IgG (I-1000) and mouse IgG (I-2000) were used as negative controls (Vector Laboratories, Burlingame, CA). The MSY2 antibody was a generous gift from Richard Schultz at the University of Pennsylvania. The Alexa Fluor® 488 goat anti-mouse IgG (A-11001) and Alexa Fluor® 488 goat anti-rabbit IgG (A-11008) secondary antibodies were purchased from ThermoFisher Scientific. The donkey anti-rabbit IgG horseradish peroxidase (HRP) (NA934) and sheep anti-mouse IgG HRP (NA931) secondary antibodies were purchased from GE Healthcare. The rabbit anti-chicken IgY (IgG) HRP (A9046) secondary antibody was purchased from Sigma-Aldrich.