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Mammocult medium kit

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The MammoCult Medium Kit is a cell culture medium designed to support the growth and expansion of mammary epithelial cells. It provides the necessary nutrients and growth factors to maintain the cells in an undifferentiated state.

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Lab products found in correlation

Vi cell xr cell viability analyzer, by Beckman Coulter (2 mentions)

Spelling variants of this product

MammoCult medium (95 citations) MammoCult™ Human Medium Kit (57 citations) MammoCult (24 citations)

2 protocols using mammocult medium kit

1

Cell Proliferation and Tumorsphere Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proliferation assays were conducted in 6-well plates in indicated medium
and manual counting of cells was performed after trypsinization at indicated
time points. Cell counting was performed using Vi-CELL XR Cell Viability
analyzer (Beckman Coulter). Tumorsphere assays were conducted using the
MammoCult Medium Kit (Stemcell Technologies; 05620) supplemented with 4ug/ml
heparin, 0.48ug/ml hydrocortisone, pen/strep, and 1% methylcellulose. 100 cells
were seeded per replicate with 4 replicates per condition and spheres were
counted on day ten.
Zhang Y., Donaher J.L., Das S., Li X., Reinhardt F., Krall J.A., Lambert A.W., Thiru P., Keys H.R., Khan M., Hofree M., Wilson M.M., Yedier-Bayram O., Lack N.A., Onder T.T., Bagci-Onder T., Tyler M., Tirosh I., Regev A., Lees J.A, & Weinberg R.A. (2022). Genome-wide CRISPR screen identifies PRC2 and KMT2D-COMPASS as regulators of distinct EMT trajectories that contribute differentially to metastasis. Nature cell biology, 24(4), 554-564.
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2

Cell Proliferation and Tumorsphere Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proliferation assays were conducted in 6-well plates in indicated medium
and manual counting of cells was performed after trypsinization at indicated
time points. Cell counting was performed using Vi-CELL XR Cell Viability
analyzer (Beckman Coulter). Tumorsphere assays were conducted using the
MammoCult Medium Kit (Stemcell Technologies; 05620) supplemented with 4ug/ml
heparin, 0.48ug/ml hydrocortisone, pen/strep, and 1% methylcellulose. 100 cells
were seeded per replicate with 4 replicates per condition and spheres were
counted on day ten.
Zhang Y., Donaher J.L., Das S., Li X., Reinhardt F., Krall J.A., Lambert A.W., Thiru P., Keys H.R., Khan M., Hofree M., Wilson M.M., Yedier-Bayram O., Lack N.A., Onder T.T., Bagci-Onder T., Tyler M., Tirosh I., Regev A., Lees J.A, & Weinberg R.A. (2022). Genome-wide CRISPR screen identifies PRC2 and KMT2D-COMPASS as regulators of distinct EMT trajectories that contribute differentially to metastasis. Nature cell biology, 24(4), 554-564.
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