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Plko lentiviral plasmid vectors of human g9a shrna and glp shrna

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The PLKO lentiviral plasmid vectors contain shRNA sequences targeting the human G9a and GLP genes. These vectors are designed for gene knockdown studies in cell lines and primary cells.

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PLKO lentiviral vector PLKO-shRNA vectors Lentiviral shRNA vectors ShRNA lentiviral vector PLKO vector Lentiviral vector ShRNA vectors PLKO shRNAs Lentiviral shRNAs Lentiviral plasmids

2 protocols using plko lentiviral plasmid vectors of human g9a shrna and glp shrna

1

Plasmid Constructs for Cyclin D1 and G9a

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The pBIND plasmids expressing GAL4-tagged cyclin D1 wild type and mutations were as described previously [56 (link)]. The pcDNA3.1 plasmids encoding FLAG-tagged G9aWT and deletion mutations were obtained from Dr. Eiji Hara (Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan) [57 (link)]. The pLKO lentiviral plasmid vectors of human G9a shRNA and GLP shRNA were purchased from Sigma.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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2

Plasmid Constructs for Cyclin D1 and G9a

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The pBIND plasmids expressing GAL4-tagged cyclin D1 wild type and mutations were as described previously (57 (link)). The pcDNA3.1 plasmids encoding FLAG-tagged G9aWT and deletion mutations were obtained from Dr. Eiji Hara (Cancer Institute, Japanese Foundation for Cancer Research, Tokyo, Japan) (58 (link)). The pLKO lentiviral plasmid vectors of human G9a shRNA and GLP shRNA were purchased from Sigma.
Li Z., Jiao X., Di Sante G., Ertel A., Casimiro M.C., Wang M., Katiyar S., Ju X., Klopfenstein D.V., Tozeren A., Dampier W., Chepelev I., Jeltsch A, & Pestell R.G. (2019). Cyclin D1 integrates G9a-mediated histone methylation. Oncogene, 38(22), 4232-4249.
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