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Ab75846

Manufactured by Abcam
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Sourced in United Kingdom

Ab75846 is a lab equipment product offered by Abcam. It serves as a core functional component for various laboratory applications. The product details and intended uses are not available in an unbiased and factual format.

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Lab products found in correlation

Nb100 105, by Novus Biologicals (1 mentions) Kgmg010w10, by Keygen Biotech (1 mentions) Anti cyclin a, by BD (1 mentions) Anti atf4, by Cell Signaling Technology (1 mentions) Anti phospho gcn2 t899, by Abcam (1 mentions) Anti cyclin d1, by BD (1 mentions) Anti p21 sc 6246, by Santa Cruz Biotechnology (1 mentions) Anti gcn2, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti p27, by BD (1 mentions) Ab38449, by Abcam (1 mentions) Ab92494, by Abcam (1 mentions) Ab40794, by Abcam (1 mentions) Ab81298, by Abcam (1 mentions) Ab179450, by Abcam (1 mentions) Ab8805, by Abcam (1 mentions) Ab8226, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

3 protocols using ab75846

1

Western Blotting of TRIB3, HIF-1α, and HIF-2α

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Total protein was extracted, quantified, and loaded onto precast PAGE gels (#KGMG010W10, KeyGEN, Jiangsu, Nanjing). After electrophoresis, proteins were transferred to nitrocellulose membranes and incubated with primary antibodies at 4°C overnight (anti-TRIB3, recombinant rabbit monoclonal, 1:7000 dilution, #ab75846, Abcam; anti-HIF-1α, mouse monoclonal, 1:1000 dilution, #NB100-105, Novus, Littleton, CO, USA; anti-HIF-2α, rabbit polyclonal, 1:1000 dilution, #NB100-122SS, Novus; β-tubulin, Rabbit monoclonal, 1:1000 dilution, #2128, Cell Signaling Technology, Danvers, MA, USA). The secondary antibody (ProteinFind Goat Anti-Rabbit/Mouse IgG(H+L), HRP conjugate, 1:1000 dilution, #HS201, TransGen, Beijing, China) was subsequently added, and immune activity was detected by chemiluminescence.
Hong B., Zhou J., Ma K., Zhang J., Xie H., Zhang K., Li L., Cai L., Zhang N., Zhang Z, & Gong K. (2019). TRIB3 Promotes the Proliferation and Invasion of Renal Cell Carcinoma Cells via Activating MAPK Signaling Pathway. International Journal of Biological Sciences, 15(3), 587-597.
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2

Immunoblotting of Cell Cycle and Stress Regulators

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Immunoblotting was carried out as previously described [48 (link)]. Commercially available antibodies used were as follws: anti-p21 (sc-6246; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p27 (610241; BD Biosciences, Franklin Lakes, NJ, USA), anti-cyclin A (611268; BD Biosciences), anti-cyclin D1 (556470; BD Biosciences), anti-ATF4 (11815; Cell Signaling Technology, Beverly, MA, USA), anti-PERK (5683; Cell Signaling Technology), anti-GCN2 (3302; Cell Signaling Technology), anti-TRB3 (ab75846; Abcam, Cambridge, UK), anti-phospho-GCN2 (T899) (ab75836; Abcam), anti-phospho-PKR (T451) (07-886; Sigma) anti-β-actin (A5441; Sigma), and anti-PKR (MAB1980; R&D Systems, Minneapolis, MN, USA).
Nishikawa S., Itoh Y., Tokugawa M., Inoue Y., Nakashima K.I., Hori Y., Miyajima C., Yoshida K., Morishita D., Ohoka N., Inoue M., Mizukami H., Makino T, & Hayashi H. (2019). Kurarinone from Sophora Flavescens Roots Triggers ATF4 Activation and Cytostatic Effects Through PERK Phosphorylation. Molecules, 24(17), 3110.
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3

Protein Expression Analysis by Western Blot

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Western blotting was performed to examine the protein level of targeted signaling molecule. The protein lysates from A549 and PC-9 cells were separated by SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, MA, USA). The membrane was incubated with the primary antibodies against to anti-p-FAK (ab81298, 1:1000, Abcam, Cambridge, UK), anti-t-FAK (ab40794, 1:1000, Abcam, Cambridge, UK), anti-p-AKT (ab38449, 1:1000, Abcam, Cambridge, UK), anti-t-AKT (ab8805, 1:1000, Abcam, Cambridge, UK), anti-FOXO1 (ab179450, 1:1000, Abcam, Cambridge, UK), anti-SOX2 (ab92494, 1:1000, Abcam, Cambridge, UK), anti-TRIB3 (ab75846, 1:1000, Abcam, Cambridge, UK) and anti-β-actin (ab8226, 1:1000, Abcam, Cambridge, UK), followed by incubation with an HRP-conjugated secondary antibody (1:1000, Abcam, Cambridge, UK).
Zhou W., Ma J., Meng L., Liu D, & Chen J. (2022). Deletion of TRIB3 disrupts the tumor progression induced by integrin αvβ3 in lung cancer. BMC Cancer, 22, 459.
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