LR73 phagocytes or BMDMs were seeded in 10 cm plates at a concentration of 2×106 cells per plate. In some experiments, apoptotic Jurkat cells were added to LR73 cells at a 10:1 ratio and incubated for the indicated times. After washing unbound Jurkat cells, the remaining cells were lysed in RIPA buffer and used in total protein Western blots or phosphorylation blots (Phos-Tag; Wako). The blots were probed using SLC12A2 (Cell Signaling Technology), SLC12A4 (Abcam), and total ERK2 (Santa Cruz Biotechnology, #sc-154-G) antibodies in Can Get Signal solution (TOYOBO) followed by chemiluminescence detection.
Slc12a4
Manufactured by Abcam
SLC12A4 is a potassium-chloride cotransporter protein that plays a role in the regulation of cellular ion homeostasis. It is responsible for the electroneutral movement of potassium and chloride ions across the cell membrane. Further details on its specific functions and applications are not available.
Automatically generated - may contain errors
2 protocols using slc12a4
1
Phagocyte SLC12A2/4 Protein Blots
2
Phagocyte SLC12A2/4 Protein Blots
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!