Ccr2rfp mice

C57BL/6J mice were purchased from Kunming Medical University. CX₃CR₁^GFP and CCR₂^RFP mice were purchased from the Jackson Laboratory (Stock No. 005582 for CX₃CR₁^GFP mouse and Stock No. 017586 for CCR₂^RFP mouse). The two lines were crossed to obtain CX₃CR₁^GFPCCR₂^RFP mice. This study was approved by the Animal Care and Use Committee of Kunming Medical University (permit# SYXK2015-0002) and was performed following the International Guiding Principles for Animals Research, as stipulated by the Council for International Organizations of Medical Sciences (1985). Mice (both C57BL/6J and CX₃CR₁^GFPCCR₂^RFP mice) and their offspring were group-housed with ad libitum access to water and food on a 12-h light/dark cycle and in a thermoregulated environment. P 9–10 mouse pups (both sexes) were used for modeling HI brain injury, and each experiment used 3–26 animals per group (the exact numbers are presented in the figures). The overall mortality was 12.5%. All efforts were made to minimize the number of mice used and their suffering.

For these studies, a total of 170 mice were used. All animal experiments were approved by the Institutional Animal Care and Use Committee at Rush University Medical Center. Animals were housed with food and water ad libitum and kept on 12-h light cycles. Wild type (WT) C57BL/6 J male mice were bred at Rush. Ccr2 null mice on a C57BL/6 J background were obtained from Taconic Farms (#3736). Pirt-GCaMP3 mice were received as a gift from Dr. Xinzhong Dong and bred at Rush. These knock-in mice express the fluorescent calcium indicator GCaMP3 in ~ 90% of all DRG sensory neurons, and not in other peripheral or central tissues, through the Pirt promoter [26 (link)]. Ccr2 null × Pirt-GCaMP3 mice were generated through two back-crosses at Rush. Ccr2^RFP mice were obtained from the Jackson Laboratory (#017586).