Ncm460

NCM460, a normal human colonic epithelial cell line, was obtained from INCELL Corporation LLC. The human colon cancer cell line HCT116 [p53 wild-type (wt)] was obtained from the Korea Cell Line Bank, and p53-null HCT116 cells were kindly provided by Dr Young-Chae Chang of the Department of Cell Biology, Catholic University of Daegu School of Medicine (Daegu, Republic of Korea). Human colon cancer DLD-1, Caco-2 and HT-29 cells were purchased from the American Type Tissue Culture Collection. The HCT116 and DLD-1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin/streptomycin (HyClone; GE Healthcare Life Sciences) at 37°C, 5% CO₂. NCM460, Caco-2 and HT-29 cells were cultured in DMEM/High glucose medium (HyClone; GE Healthcare Life Sciences) supplemented in the same manner as the RPMI-1640.

The human colon cancer cell line SW480 and human oxaliplatin-resistant colon cancer cell line SW480-OXA were purchased from the Shanghai Institute of Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and the normal human colon epithelial cell line NCM460 was obtained from INCELL. The mouse colon cancer cell line CT26 was purchased from the Shanghai Institute of Cell Biology of the Chinese Academy of Sciences (Shanghai, China). CT26, SW480, and SW480-OXA cells were cultured in RPMI 1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) with routine addition of ampicillin and streptomycin. NCM460 cells were cultured in DMEM/high-glucose medium (HyClone, GE Healthcare Life Sciences) supplemented in the same manner as for RPMI 1640.