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Anti thy1 fitc 5e10

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Anti-THY1-FITC (5E10) is a mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC). It is designed to detect the expression of the Thy-1 (CD90) cell surface antigen.

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Lab products found in correlation

Anti cd45 apc h7 2d1, by BD (2 mentions) Anti cd31 pe cyanine7 wm 59, by Thermo Fisher Scientific (2 mentions) Anti cd34 pe 4h11, by Thermo Fisher Scientific (2 mentions) Anti pdpn percp efluor710 nz 1, by Thermo Fisher Scientific (2 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Streptavidin apc, by Jackson ImmunoResearch (1 mentions) Anti cd34 ep373y, by Abcam (1 mentions) Anti pdpn nz 1, by Thermo Fisher Scientific (1 mentions) Thy 1a1, by R&D Systems (1 mentions) Anti ki 67 16a8, by BioLegend (1 mentions) Anti mouse igg1 fitc, by Southern Biotech (1 mentions) Alexa fluor 647 anti rat igg, by Thermo Fisher Scientific (1 mentions) Anti fitc alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti cd29 apc, by BioLegend (1 mentions) Human trustain fcx, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-Thy1.2

2 protocols using anti thy1 fitc 5e10

1

Synovial Cell Immunophenotyping by Flow Cytometry

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The following antibodies and reagents were used for the analysis of synovial cells with flow cytometry and cell sorting: anti-CD45-APC-H7 (2D1, BD Pharmingen), anti-CD235a-APC-Alexa Fluor750 (11E4B-7-6(KC16), Beckman Coulter), anti-CD31-PE-Cyanine7 (WM-59, eBioscience), anti-CD146-BV450 (P1H12, BD Horizon), anti-CD34-PE (4H11, eBioscience), anti-PDPN-PerCP-eFluor710 (NZ-1.3, eBioscience), anti-THY1-FITC (5E10, BD Pharmingen), anti-cadherin-11-biotin (23C6), human TruStain FcX (BioLegend), streptavidin-APC (Jackson ImmunoResearch), Live/Dead fixable aqua dead cell stain kits (Molecular Probes). For immunofluorescence staining of synovial tissue, following antibodies and reagents were used: anti-CD45 (135-4C5, AbD Serotec), anti-CD34 (EP373Y, Abcam), anti-PDPN (NZ-1.3, eBioscience), anti-THY1 (F15-42-1, Merck Millipore, and clone Thy-1A1, R&D Systems), anti-cadherin-11-Biotin (23C6), anti-Ki67 (16A8, BioLegend), anti-mouse IgG1-FITC (Southern Biotech), anti-mouse IgG2a FITC (Southern Biotech), anti-mouse IgG2b-Alexa Fluor 647 (Life Technologies), anti-rat IgG-Alexa Fluor 594 (Life Technologies), anti-rat IgG-Alexa Fluor 647, anti-rabbit IgG-Alexa Fluor 546 (Life Technologies), Hoechst 33258 (Life Technologies), and anti-FITC Alexa Fluor 488 (Life Technologies).
Mizoguchi F., Slowikowski K., Wei K., Marshall J.L., Rao D.A., Chang S.K., Nguyen H.N., Noss E.H., Turner J.D., Earp B.E., Blazar P.E., Wright J., Simmons B.P., Donlin L.T., Kalliolias G.D., Goodman S.M., Bykerk V.P., Ivashkiv L.B., Lederer J.A., Hacohen N., Nigrovic P.A., Filer A., Buckley C.D., Raychaudhuri S, & Brenner M.B. (2018). Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis. Nature Communications, 9, 789.
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2

Synovial Cell Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies and reagents were used for the analysis of synovial cells with flow cytometry and cell sorting: anti-CD45-APC-H7 (2D1, BD Biosciences, CA, USA), anti-CD235a-APC-Alexa Fluor750 (11E4B-7-6, Beckman Coulter, FL, USA), anti-CD31-PE-Cyanine7 (WM-59, eBioscience, CA, USA), anti-CD146-APC (P1H12, eBioscience), anti-CD34-PE (4H11, eBioscience), anti-PDPN-PerCP-eFluor710 (NZ-1.3, eBioscience), anti-THY1-FITC (5E10, BD Bioscience), anti-CD73-PE-CF594 (AD2, BD Bioscience), anti-CD271-APC (ME20.4, eBioscience), anti-CD54-PE-CF594 (HA58 BioLegend, CA, USA), anti-CD44-APC (G44-26 BD Bioscience), anti-CD29-APC (TS2/16 BioLegend), human TruStain FcX (BioLegend), and Live/Dead fixable aqua dead cell stain kits (Molecular Probes, Thermo Fisher Scientific, MA, USA).
Noda S., Hosoya T., Komiya Y., Tagawa Y., Endo K., Komori K., Koga H., Takahara Y., Sugimoto K., Sekiya I., Saito T., Mizoguchi F, & Yasuda S. (2022). CD34+THY1+ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro. Arthritis Research & Therapy, 24, 45.
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