Log In Sign up
The largest database of trusted experimental protocols

Rag B is a laboratory antibody product offered by Cell Signaling Technology. It is used to detect the Rag B protein in various biological samples. Rag B is a member of the Rag GTPase family and plays a role in regulating the activity of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Rapamycin, by Merck Group (2 mentions) Pras40, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) Hygromycin b, by Corning (2 mentions) Puromycin antibody, by Kerafast (2 mentions) C flip antibody, by Enzo Life Sciences (2 mentions) Hexadecylsulfonylfluoride hdsf, by Santa Cruz Biotechnology (2 mentions) P s6 s235 6, by Cell Signaling Technology (2 mentions) β tubulin antibody, by Merck Group (2 mentions) Tw 37, by Selleck Chemicals (2 mentions) C myc, by Santa Cruz Biotechnology (2 mentions) Mg132, by Merck Group (2 mentions) Cleaved parp, by Cell Signaling Technology (2 mentions) Bafilomycin a1, by Merck Group (2 mentions) Mcl 1, by Cell Signaling Technology (2 mentions) Puromycin, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) P 4ebp1 s65, by Cell Signaling Technology (1 mentions) P s6kt389, by Cell Signaling Technology (1 mentions)

3 protocols using rag b

1

Comprehensive Antibody Reagents for Cellular Signaling Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against pS6K (T389) (#9205), S6K (#9202), pS6 (S240/244) (#2215), S6 (#2217), pAkt (T308) (#13038), pAkt (S473) (#4060), AKT (4691), p4E-BP1 (S65) (#9456), 4E-BP1 (#9644), RagA (#4357), RagB (#8150), RagC (#9480), Sestrin2 (#8487), MIOS (#13557), EEA1 (#3288, used for immunofluorescence (IF)), Lamp1 (#9091, used for IF) and Rab7 (#9367, used for IF) were purchased from Cell Signaling Technology. Lamp2 mouse monoclonal antibody (H4B4, for human cells) and Lamp2 rat-monoclonal antibody (GL2A7, for mouse cells) for IF were obtained from Abcam. Anti-FLAG (F1804, used for IF), anti-HA (3F10), and anti-PMP70 (SAB4200181, used for IF) were obtained from Sigma. WDR24 (20778-1-AP, used for IP) and Sestrin2 (10795-1-AP, used for IP) antibody was purchased from Proteintech. Antibodies against SZT2 (sc-242153), NPRL2 (sc-376986), SEC13 (sc-514308) and WDR59 (sc-137927, only used in immunoblotting of WDR59 in MEFs) were obtained from Santa Cruz. SEH1L (ab187307) antibody was form Abcam. The rabbit WDR59 monoclonal antibody used in IF experiments (1:100, validated in this study with knockout cells, Extended Data Fig. 5b) and immunoblotting experiments (1:1000) in HEK293T was kindly provided by Jianxin Xie from Cell Signaling Technology. This antibody only recognized WDR59 from human cells, but not MEFs. Anti-NPRL3 (NBP1-88447, used for IP) was obtained from Novus Biologicals.
Peng M., Yin N, & Li M.O. (2017). SZT2 Dictates GATOR Control of mTORC1 Signaling. Nature, 543(7645), 433-437.
+ Open protocol
+ Expand
2

Comprehensive Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
+ Open protocol
+ Expand
3

Comprehensive Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies recognizing pan-AKT, p-AKT (S473), GSK3β, p-GSK3β (S9), TSC2, p-TSC2 (T1462), S6K, p-S6K (T389), S6, p-S6 (S235/6), PRAS40, ULK1, Rag A, Rag B, Rag C, AMPK, cleaved PARP, and Mcl-1 were from Cell Signaling Technology. β-tubulin antibody was from Sigma-Aldrich. XPB (TFIIH p89 S-19), p53, c-Myc, and GFP antibodies were from Santa Cruz. puromycin antibody was from KeraFAST. REDD1/DDIT4 antibodies were from Novus and Bethyl. C-FLIP antibody was from Enzo Life Sciences. All antibodies were used at final concentrations of 1:1000 – 1:2000 except for cleaved PARP, c-Myc, p-TSC2, ULK1, and XPB (1:500), AMPK, P70S6K, and TSC2 (1:750), and β-tubulin, S6, and p-S6 (1:4000). DMSO, cycloheximide, puromycin, rapamycin, bafilomycin A1, and MG-132 were from Sigma-Aldrich. Hygromycin B was from Corning. Hexadecylsulfonylfluoride (HDSF) was from Santa Cruz. TW-37 was from Selleckchem. Torin1 was a kind gift from David Sabatini and Nathanael Gray. Smac mimetic was a kind gift from Xiaodong Wang.
Potts M.B., McMillan E.A., Rosales T.I., Kim H.S., Ou Y.H., Toombs J.E., Brekken R.A., Minden M.D., MacMillan J.B, & White M.A. (2015). Mode of action and pharmacogenomic biomarkers for exceptional responders to didemnin B. Nature chemical biology, 11(6), 401-408.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!