Pe conjugated anti mrgprx2 antibody

Manufactured by BioLegend

Sourced in United States

The PE-conjugated anti-MRGPRX2 antibody is a reagent used for the detection and analysis of the MRGPRX2 receptor in biological samples. It is a conjugate of a phycoerythrin (PE) dye and an antibody specific to the MRGPRX2 protein.

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Lab products found in correlation

P nitrophenyl n acetyl β d glucosamine pnag, by Merck Group (2 mentions) Pertussis toxin ptx, by List Biological Laboratories (1 mentions) Amaxa transfection kit kit 5, by Lonza (1 mentions) Ym 254890, by Fujifilm (1 mentions) Substance p, by AnaSpec (1 mentions) Fura 2 acetoxymethyl ester, by Abcam (1 mentions) Preceiver mo6 vector, by GeneCopoeia (1 mentions) Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions) Icatibant, by AnaSpec (1 mentions) Hrp labeled goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Supersignal west pico maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Nateglinide, by Bio-Techne (1 mentions) Compound 48 80, by AnaSpec (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

2 protocols using pe conjugated anti mrgprx2 antibody

Characterization of MRGPRX2 Signaling

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All cell culture reagents were obtained from Invitrogen (Gaithersburg, MD, USA). Amaxa transfection kit (Kit V) was obtained from Lonza (Gaithersburg, MD, USA). Q5 Site-Directed Mutagenesis Kit was from New England BioLabs (Ipswich, MA). Substance P (SP) was from AnaSpec (Fremont, CA, USA). Pertussis toxin (PTx) was from List Biological Laboratories (Campbell, CA, USA). YM-254890 was from Wako Chemicals (Richmond, VA, USA) and p-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) was from Sigma-Aldrich (St. Louis, MO, USA). Fura-2 acetoxymethyl ester was from Abcam (Cambridge, MA, USA). PE-conjugated anti-MRGPRX2 antibody was from BioLegend (San Diego, CA, USA). MRGPRX2 plasmid encoding hemagglutinin (HA)-tagged human MRGPRX2 in pReceiver-MO6 vector was obtained from GeneCopoeia (Rockville, MD, USA).

Chompunud Na Ayudhya C., Roy S., Alkanfari I., Ganguly A, & Ali H. (2019). Identification of Gain and Loss of Function Missense Variants in MRGPRX2’s Transmembrane and Intracellular Domains for Mast Cell Activation by Substance P. International Journal of Molecular Sciences, 20(21), 5247.

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MRGPRX2-mediated Activation Assay

Cited 1 time

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All cell culture and Lipofectamine 2000 transfection reagents were purchased from Invitrogen Life Technologies (Carlsbad, CA); recombinant human stem cell factor cytokine (hSCF) was from Peprotech (Rocky Hill, NJ); p-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) was from Sigma-Aldrich (St. Louis, MO); PE conjugated anti-MRGPRX2 antibody was from Biolegend (San Diego, CA); polyclonal MRGPRX2 antibody was purchased from Novus Biologicals (Littleton, CO); PE conjugated anti-FLAG antibody, HRP labeled goat anti-rabbit IgG and β-actin antibodies were obtained from Cell Signaling Technology (Danvers, MA); SuperSignal® West PicoMaximum Sensitivity Substrate was from Thermo Scientific (Rockford, IL); Compound 48/80, and Icatibant were from AnaSpec (Fremont, CA); Nateglinide was from Tocris Biosciences and AG-30/5C was from Peptide International Inc. (Louisville, KY), respectively. MRGPRX2-Tango (Addgene #66440) and MRGPRX4-Tango (Addgene#66442) plasmids were gifts from Bryan Roth (32 (link)).

Roy S., Ganguly A., Haque M, & Ali H. (2019). Angiogenic Host Defense Peptide AG-30/5C and Bradykinin B2 receptor antagonist Icatibant are G protein-biased agonists for MRGPRX2 in mast cells. Journal of immunology (Baltimore, Md. : 1950), 202(4), 1229-1238.

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