Deuterated internal standards

Manufactured by Merck Group

Sourced in United States

Deuterated internal standards are stable isotope-labeled compounds used in analytical chemistry and biochemistry to quantify analytes in complex matrices. They serve as internal references to improve the accuracy and precision of analytical measurements.

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Lab products found in correlation

Hplc grade methanol, by Merck Group (1 mentions) N hexane, by Merck Group (1 mentions) Xevo tq s, by Waters Corporation (1 mentions) Acquity ultra performance lc system, by Waters Corporation (1 mentions) Acquity beh c18 column, by Waters Corporation (1 mentions) Bile acid standards, by Merck Group (1 mentions) Pcb 126, by Cambridge Isotopes (1 mentions) Ammonium formate, by Merck Group (1 mentions) Methyl tert butyl ether (mtbe), by Merck Group (1 mentions) Leucine enkephalin, by Merck Group (1 mentions) Formic acid, by Biosolve (1 mentions) Acetonitrile, by Biosolve (1 mentions) Chloroform, by Biosolve (1 mentions)

Spelling variants of this product

Internal standards (5 citations) Deuterated standards (2 citations)

3 protocols using deuterated internal standards

Quantitative Analysis of Free Fatty Acids

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Reference standards of FFAs (purity>90%) and deuterated internal standards (IS) were acquired from Sigma-Aldrich and NU-CHEK. HPLC-grade methanol, n-hexane, acetonitrile, and water were obtained from Merck.
A Waters ACQUITY ultraperformance LC system equipped with a binary solvent delivery manager and a sample manager (Waters, Milford, MA, USA) was used throughout the study. The mass spectrometer was a Waters XEVO TQ-S instrument with an ESI source (Waters, Milford, MA, USA). The entire LC−MS system was controlled by MassLynx 4.1 software. All chromatographic separations were performed with an ACQUITY BEH C18 column (1.7 μm, 100 mm × 2.1 mm internal dimensions; Waters, Milford, MA, USA).

Yang X., Sun L., Zhao A., Hu X., Qing Y., Jiang J., Yang C., Xu T., Wang P., Liu J., Zhang J., He L., Jia W, & Wan C. (2017). Serum fatty acid patterns in patients with schizophrenia: a targeted metabonomics study. Translational Psychiatry, 7(7), e1176-.

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Isotopic Labeling for Bile Acid Analysis

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Sodium 3-(trimethylsilyl) [2,2,3,3-²H₄] propionate (TSP), D₂O (99.9% in D), and PCB 126 were ordered from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Bile acid standards and deuterated internal standards were obtained from Sigma-Aldrich (St Louis, MO, USA) and Cayman Chemical (Ann Arbor, MI, USA).

Tian Y., Rimal B., Gui W., Koo I., Smith P.B., Yokoyama S, & Patterson A.D. (2022). Early Life Polychlorinated Biphenyl 126 Exposure Disrupts Gut Microbiota and Metabolic Homeostasis in Mice Fed with High-Fat Diet in Adulthood. Metabolites, 12(10), 894.

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Comprehensive Analytical Grade Solvent Acquisition

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Analytical grade solvents acetonitrile (MeCN), methanol (MeOH), ethanol (EtOH), chloroform and formic acid were purchased from Biosolve (Biosolve BV, Valkenswaard, The Netherlands), whereas ammonium formate, methyl tert-butyl ether (MTBE), and leucine-enkephalin were purchased from Sigma–Aldrich (Sigma–Aldrich, Burlington, WV, USA). Ultrapure water was obtained from an arium pro UF/VF water purification system with a Sartopore 0.2 μm filter (Sartorius Stedim, Amersfoort, The Netherlands). The majority of standards and deuterated internal standards were purchased from Sigma Aldrich (Sigma-Aldrich, Zwijndrecht, The Netherlands), Avanti (Avanti Polar Lipids, Alabaster, AL, USA), and Fluka (Honeywell, Netherlands). More detailed information regarding the standard suppliers and utilized concentration can be found in the Supplementary Information (Supplementary Table S1).

Hosseinkhani F., Dubbelman A.C., Karu N., Harms A.C, & Hankemeier T. (2021). Towards Standards for Human Fecal Sample Preparation in Targeted and Untargeted LC-HRMS Studies. Metabolites, 11(6), 364.

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