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Tcf lef h2b gfp

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The TCF/Lef:H2B-GFP is a fluorescent fusion protein that contains the core DNA-binding domain of the T-cell factor/lymphoid enhancer factor (TCF/Lef) family of transcription factors and the histone H2B protein fused to green fluorescent protein (GFP). This fusion protein localizes to the nucleus and can be used to monitor the activity of the Wnt/β-catenin signaling pathway.

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Lab products found in correlation

Smoflox flox, by Jackson ImmunoResearch (2 mentions) Gli1 lacz, by Jackson ImmunoResearch (2 mentions) Rosa26mtmg, by Jackson ImmunoResearch (2 mentions) Krt14 cre, by Jackson ImmunoResearch (2 mentions) Gt rosa 26sortm14 cag tdtomato tdtomato reporter line, by Jackson ImmunoResearch (1 mentions) Cdh5 cre, by Jackson ImmunoResearch (1 mentions) Flk1 gfp, by Jackson ImmunoResearch (1 mentions) Wnt5aflox flox mice, by Jackson ImmunoResearch (1 mentions) Tg teto hist1h2bj gfp 47efu j, by Jackson ImmunoResearch (1 mentions) Bat gal b6 cg tg bat lacz 3picc j, by Jackson ImmunoResearch (1 mentions) Axin2lacz, by Jackson ImmunoResearch (1 mentions) Rosacreert2, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

TCF/Lef:H2B/GFP mice (6 citations) TCF/Lef:H2B-GFP transgenic mice (3 citations) TCF/LEF:H2B-GFP reporter line (2 citations)

6 protocols using tcf lef h2b gfp

1

Transgenic Mouse Models for Cellular Visualization

Cited 4 times
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Keratin19CreER mice (31 (link)) were provided by G. Gu. β-catnflox(Ex3)/+ mice (32 (link)) were bred to K19CreER mice to generate K19CreER;β-catnflox(Ex3)/+ mice. K14-H2BGFP (33 (link)) and Lef1-RFP (7 (link)) transgenic mice were used for visualization of epithelial and mesenchymal cells with the 2-photon microscope. Wntlessfl/fl mice (34 (link)), TcfLef-H2BGFP (35 (link)), Gt(ROSA)26Sortm14(CAG-tdTomato) (tdTomato reporter line) (24 (link)) were obtained from Jackson Laboratories. All procedures involving animal subjects were performed under the approval of the Institutional Animal Care and Use Committee (IACUC) of the Yale School of Medicine.
Deschene E.R., Myung P., Rompolas P., Zito G., Sun T.Y., Taketo M.M., Saotome I, & Greco V. (2014). β-catenin activation regulates tissue growth non-cell autonomously in the hair stem cell niche. Science (New York, N.Y.), 343(6177), 1353-1356.
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2

Lineage Tracing of Cardiac Endothelial Cells

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All procedures were approved by the Institutional Animal Care and Use Ethics Committee of Weill Cornell Medical College. Breeding was performed using animals between 12 weeks and 1 year of age. The mouse strains that were intercrossed to generate the animals in this study were: Flk1-GFP (Jackson Labs, 017006), RosatdTom (Jackson Labs, 007905), TCF:LEFH2B-GFP (Jackson Labs, 013752), Cdh5Cre (Jackson Labs, 017968), β-cateninflox/flox (Jackson Labs, 004152), Igs1tm6(CAG-Bgeo,-Wnt5a)Nat (Wnt5aGOF; Jackson Labs, 018141), Wnt5aflox/flox mice (Jackson Labs, 026626), Nkx2.5Cre [provided by Richard Harvey (Stanley et al., 2002)] and β-cateninflEx3/flEx3 [provided by Makoto Taketo (Harada et al., 1999)]. For quantification of NkxEC versus nEC number, 10 µm sections were collected for the entire span of the heart and colocalization masking distinguished EC subtypes.
Reichman D.E., Park L., Man L., Redmond D., Chao K., Harvey R.P., Taketo M.M., Rosenwaks Z, & James D. (2018). Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development (Cambridge, England), 145(1), dev159905.
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3

Lineage Tracing in Developing Mice

Cited 1 time
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All mice used for this study were housed at the Center of Comparative Medicine and Surgery (CCMS), Icahn School of Medicine at Mount Sinai (ISMMS), according to the Institutional Animal Care and Use Committee (IACUC) guidelines (Protocol number LA11-0020). Krt14-Cre, Smoflox/flox, Gli1lacZ, TCF/Lef:H2B-GFP and Rosa26-mT/mG mice were obtained from the Jackson Laboratories. Eedflox/flox mice were kindly provided by Weipeng Mu and Terry Magnuson (Mu et al., 2014 (link)). Ink4a/Arf−/− mice were kindly provided by Philippe Soriano (Serrano et al., 1996 (link)). Both male and female mice were used in this study. Primer sequences used for genotyping are available in Table S4. BrdU was administered to newborn P0 mice by sub-cutaneous injection (50μg per 1g of mouse weight) 4 hours before sacrifice.
Bar C., Cohen I., Zhao D., Pothula V., Litskevitch A., Koseki H., Zheng D, & Ezhkova E. (2019). Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell reports, 28(1), 257-266.e5.
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4

Naphthalene-Induced Lung Injury in Mice

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Experiments involving mice were performed according to protocols approved by the Institutional Animal Care and Use Committee of the University of Iowa. Wild-type C57BL/6, BAT-gal (B6.Cg-Tg(BAT-lacZ)3Picc/J), TCF/Lef:H2B-GFP (TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J), Rosa26-FlxTomatoStopFlx-eGFP (Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J), and Rosa26-rtTA/TetON:H2B-GFP (B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J) crossed with Tg(tetO-HIST1H2BJ/GFP)47Efu/J) mouse strains were purchased from The Jackson Laboratory. Mice were injured with a single IP injection of 300 μg Naphthalene per gram bodyweight and pulsed with nucleotide labels as described in the Supplemental Methods.
Lynch T.J., Anderson P.J., Xie W., Crooke A.K., Liu X., Tyler S.R., Luo M., Kusner D.M., Zhang Y., Neff T., Burnette D.C., Walters K.S., Goodheart M.J., Parekh K.R, & Engelhardt J.F. (2016). WNT SIGNALING REGULATES AIRWAY EPITHELIAL STEM CELLS IN ADULT MURINE SUBMUCOSAL GLANDS. Stem cells (Dayton, Ohio), 34(11), 2758-2771.
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5

Conditional Gfi1b Deletion in Megakaryocytes

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Gfi1bfl/fl mice9 (link) were crossed with Rosa-CreERT2, and Axin2+/LacZ (Jackson, B6.129P2-Axin2tm1Wbm/J) or TCF/Lef:H2B-GFP (Jackson, 61Hadj/J) transgene reporter line. PF4-cre mice were used to delete Gfi1b in MKs for immunofluorescence microscopy analysis. Age and sex-matched mice were used in all experiments. For BM transplantation C57B6 CD45.1 mice were used. All mice were housed under SPF conditions and the IRCM Institutional Review Board approved all animal protocols and experimental procedures were performed in compliance with IRCM and CCAC (Canadian Council of Animal Care) guidelines.
Shooshtarizadeh P., Helness A., Vadnais C., Brouwer N., Beauchemin H., Chen R., Bagci H., Staal F.J., Coté J.F, & Möröy T. (2019). Gfi1b regulates the level of Wnt/β-catenin signaling in hematopoietic stem cells and megakaryocytes. Nature Communications, 10, 1270.
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6

Lineage Tracing in Developing Mice

Cited 1 time
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All mice used for this study were housed at the Center of Comparative Medicine and Surgery (CCMS), Icahn School of Medicine at Mount Sinai (ISMMS), according to the Institutional Animal Care and Use Committee (IACUC) guidelines (Protocol number LA11-0020). Krt14-Cre, Smoflox/flox, Gli1lacZ, TCF/Lef:H2B-GFP and Rosa26-mT/mG mice were obtained from the Jackson Laboratories. Eedflox/flox mice were kindly provided by Weipeng Mu and Terry Magnuson (Mu et al., 2014 (link)). Ink4a/Arf−/− mice were kindly provided by Philippe Soriano (Serrano et al., 1996 (link)). Both male and female mice were used in this study. Primer sequences used for genotyping are available in Table S4. BrdU was administered to newborn P0 mice by sub-cutaneous injection (50μg per 1g of mouse weight) 4 hours before sacrifice.
Bar C., Cohen I., Zhao D., Pothula V., Litskevitch A., Koseki H., Zheng D, & Ezhkova E. (2019). Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell reports, 28(1), 257-266.e5.
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