Venous blood samples were collected immediately after exercise, and blood analysis was performed using whole blood and serum. To collect the serum, the venous blood samples were allowed to clot at 24–25 °C for 30 min, centrifuged at 2000× g for 15 min at 4 °C, and transferred to new tubes before being stored at −80 °C. Whole blood was used to measure the concentration of blood glucose (ACCU CHEK Performa Glucometer, Roche, Diagnostics, Penzberg, Germany), lactate (Lactate Pro2, LT-1730, ARKRAY, Kyoto, Japan), and TG (Standard LipidoCare Strips–Lipid Profile, 02LA10G, SD Biosensor, Suwon, Korea). Serum was used in ELISA kits for the analysis of the concentration of glycerol (EGLY-200, BioAssay System, Hayward, CA, USA) and FFAs (K612-100, BioVision, Milpitas, CA, USA).
Egly 200
Manufactured by BioAssay Systems
Sourced in United States
The EGLY-200 is a laboratory instrument designed for electrochemical detection. It is capable of measuring the electrical properties of various samples, including solutions and solid materials. The EGLY-200 provides precise and reliable measurements, making it a valuable tool for researchers and scientists in various fields of study.
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Lab products found in correlation
2 protocols using egly 200
1
Venous Blood Analysis After Exercise
2
Plasma Lipid and Glycemic Biomarkers
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Plasma triglycerides (TG) were measured with an enzymatic colorimetric commercial assay kit (ETGA-200 and EGLY-200, BioAssay Systems). Total cholesterol, combined low-density lipoprotein cholesterol (LDL-C), and very low density lipoprotein cholesterol (VLDL-C), as well as high-density lipoprotein cholesterol (HDL-C), were measured using an enzymatic colorimetric commercial assay kit (EHDL-100, BioAssay Systems). In the animals undergoing CEUMI, plasma HbA1c was measured using a sandwich enzyme-linked immunosorbent commercial assay kit (E4657-100, BioVision), and nonfasting plasma glucose was measured using mouse glucose assay kit (#81692, Crystal Chem) in blood drawn after the imaging studies. In a separate group of animals (n = 20 on liraglutide treatment and n = 20 on vehicle treatment), whole-blood HbA1c was measured at baseline, day 44, and day 80 in a 5-μL full blood sample taken from the tip of the tail by puncturing the capillary bed with a lancet, using a heparinized capillary tube to sample the blood. The capillary tube was then shaken into 500 μL of Hitachi Hemolyzing Reagent and measured in a Hitachi 912 autoanalyzer (Roche A/S Diagnostics), according to the manufacturer’s instructions. Assays were performed in duplicates according to manufacturer’s protocol with the exception of whole-blood HbA1c assays, which were done as single measurements.
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