Cd3 e780

Flow cytometry antibodies: B220/CD45R-e450, CD3-e780, CD11b-APC, CD11c-e780, CD19-e780, CD49b-e780 CD86-Alexa Fluor 488, NK1.1-e780 from eBioscience. CD8α-PECF594, CD40-PE from BD Pharmingen. B220-BV650, CD11b-BV711, CD11c-BV421, CD40-FITC, CD80-BV605, CD86-A700, F4/80-PE-Cy7, Ly6C-BV570, Ly6G-APC-Cy7, MHC II-PerCP-Cy5.5 from Biolegend. PDCA-1-PE, FcR block from Miltenyi Biotec. The S9.6 monoclonal antibody against RNA:DNA hybrids was purified from hybridoma cell line HB-8730 (ATCC-LGC Promochem) supernatant using a Protein A/G column as previously described (Pohjoismaki et al, 2010 (link)).

For immunofluorescence staining of whole blood and PBMC, antibodies included CD45-ECD (Beckman Coulter, Indianapolis IN), CD3-e780, CD4-e450, CD8-AF700, CD15-FITC (eBioscience, San Diego, CA), CD45-FITC, CD4-PerCP-Cy5-5, CD14-PECy7, HLA-DR-APC, HLA-DR-BV421, CD8-APC-H7, CCR7-BV605, CD25-BV605, CD45RA-PECy7, CD45RO-APC-H7, CCR4-PECy7, CD38-APC, CD127-BV650 (BD Bioscience, San Jose, CA), CD14-AF700, and CD3-AF700 (Biolegend, San Diego, CA). For phosphoSTAT staining, antibodies included CD3-BV785 (Biolegend), CD4-BV605, CD8-BV510, CD14-PECy7, CD19-BV421, CD16-BV650, pSTAT3-647, pSTAT5-PE (BD Biosciences), and pSTAT1-488 (Cell Signaling Technologies, Danvers, MA). Cell types were identified according to the criteria of the Human Immunology Project [19 (link)], and a full list of antibodies used to identify individual cell types including those not reported here can be found in Additional file 2: Table S1. Hematological toxicity was graded according to NCI CTCAE v4.0 guidelines and is reported in Additional files 3 and 4: Tables S2 and S3.