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GM03529 cells are a fibroblast cell line derived from a healthy male donor. They are a commonly used reference cell line for various research applications.

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2 protocols using gm03529 cells

1

Cell Line Authentication and Maintenance

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Human BJ dermal fibroblasts (CRL-2522) and HEK293T/17 (CRL-11268) cells were purchased from ATCC and maintained in DMEM supplemented with 10% FBS, NEAA, and β-ME. GM03529 cells were obtain from Coriell Institute and maintained in DMEM supplemented 15% FBS, NEAA, and β-ME. WA09 hESCs were from WiCell Institute and maintained in NutriStem® hPSC XF medium. No cell lines used in this study are listed in the International Cell Line Authentication Committee (ICLAC) Register of Misidentified Cell Lines or the NCBI Biosample database of misidentified cell lines. All cell lines were authenticated by Interspecies Determination (Isoenzyme Analysis and STR analysis) by the providing company and were routinely tested for mycoplasma detection using a Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich).
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2

Cell Line Authentication and Maintenance

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human BJ dermal fibroblasts (CRL-2522) and HEK293T/17 (CRL-11268) cells were purchased from ATCC and maintained in DMEM supplemented with 10% FBS, NEAA, and β-ME. GM03529 cells were obtain from Coriell Institute and maintained in DMEM supplemented 15% FBS, NEAA, and β-ME. WA09 hESCs were from WiCell Institute and maintained in NutriStem® hPSC XF medium. No cell lines used in this study are listed in the International Cell Line Authentication Committee (ICLAC) Register of Misidentified Cell Lines or the NCBI Biosample database of misidentified cell lines. All cell lines were authenticated by Interspecies Determination (Isoenzyme Analysis and STR analysis) by the providing company and were routinely tested for mycoplasma detection using a Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich).
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