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Cls431750 50ea

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The CLS431750-50EA is a laboratory equipment product. It is a 50-pack of sterile disposable cell culture-treated culture flasks. The flasks are designed for adherent cell culture applications.

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Lab products found in correlation

Vitronectin xf coated, by STEMCELL (3 mentions) Am9260g, by Thermo Fisher Scientific (3 mentions) Ab0626, by Thermo Fisher Scientific (3 mentions) Accutase, by Merck Group (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)

3 protocols using cls431750 50ea

1

Feeder-Free Culture of hiPSCs

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Human-induced pluripotent stem cells (hiPSCs) were thawed and cultured under feeder-free conditions on Vitronectin XF™-coated (Stem Cell, #07180) 10 cm tissue culture treated plates (Corning) in complete Essential 8 medium (Life Technologies #A1517001) supplemented with 1% Penicillin/Streptomycin (Invitrogen, #15140122). Cells were incubated at 37°C, 5% CO2 and media changed every day except for the day of passaging. Cells were routinely passaged using 0.5 mM EDTA (Life Technologies #AM9260G) at least 3 times post-thawing before collection. HiPSCs were harvested using Accutase (Millipore, #SCR005) to generate a single cell suspension. Single cells were resuspended in 1X PBS (Thermo Fisher Scientific, #10010023) and passed through a 40uM filter (Corning #CLS431750-50EA) before FAC sorting (BD Influx™ Cell Sorter) into 96-well plates (Framestar, #4TI-0960) containing 2µl lysis buffer with 1U/µl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626), spun down and frozen at -80 °C until further processing.
Berrens R.V., Yang A., Laumer C., Lun A.T.L., Bieberich F., Law C., Lan G., Imaz M., Gaffney D.J., & Marioni J.C. (2020). Transposable element expression at unique loci in single cells with CELLO-seq.
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2

Single-cell RNA-seq of hiPSCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human-induced pluripotent stem cells (hiPSCs) (https://www.hipsci.org/lines/#/lines/HPSI0714i-nufh_3, https://www.hipsci.org/lines/#/lines/HPSI0914i-euts_1)59 (link) were thawed and cultured under feeder-free conditions on Vitronectin XF™-coated (Stem Cell, #07180) 10 cm tissue culture treated plates (Corning) in complete Essential 8 medium (Life Technologies #A1517001) supplemented with 1% Penicillin/Streptomycin (Invitrogen, #15140122). Cells were incubated at 37°C, 5% CO2 and media changed every day except for the day of passaging. Cells were routinely passaged using 0.5 mM EDTA (Life Technologies #AM9260G) at least 3 times post-thawing before collection. HiPSCs were harvested using Accutase (Millipore, #SCR005) to generate a single cell suspension. Single cells were resuspended in 1X PBS (Thermo Fisher Scientific, #10010023) and passed through a 40uM filter (Corning #CLS431750-50EA) before FAC sorting (BD Influx™ Cell Sorter) into 96-well plates (Framestar, #4TI-0960) containing 2 μl lysis buffer with 1 U/μl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626), spun down and frozen at -80 °C until further processing.
Berrens R.V., Yang A., Laumer C.E., Lun A.T., Bieberich F., Law C.T., Lan G., Imaz M., Bowness J.S., Brockdorff N., Gaffney D, & Marioni J.C. (2021). Locus-specific expression of transposable elements in single cells with CELLO-seq. Nature biotechnology, 40(4), 546-554.
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3

Single-cell RNA-seq of hiPSCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human-induced pluripotent stem cells (hiPSCs) (https://www.hipsci.org/lines/#/lines/HPSI0714i-nufh_3, https://www.hipsci.org/lines/#/lines/HPSI0914i-euts_1)59 (link) were thawed and cultured under feeder-free conditions on Vitronectin XF™-coated (Stem Cell, #07180) 10 cm tissue culture treated plates (Corning) in complete Essential 8 medium (Life Technologies #A1517001) supplemented with 1% Penicillin/Streptomycin (Invitrogen, #15140122). Cells were incubated at 37°C, 5% CO2 and media changed every day except for the day of passaging. Cells were routinely passaged using 0.5 mM EDTA (Life Technologies #AM9260G) at least 3 times post-thawing before collection. HiPSCs were harvested using Accutase (Millipore, #SCR005) to generate a single cell suspension. Single cells were resuspended in 1X PBS (Thermo Fisher Scientific, #10010023) and passed through a 40uM filter (Corning #CLS431750-50EA) before FAC sorting (BD Influx™ Cell Sorter) into 96-well plates (Framestar, #4TI-0960) containing 2 μl lysis buffer with 1 U/μl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626), spun down and frozen at -80 °C until further processing.
Berrens R.V., Yang A., Laumer C.E., Lun A.T., Bieberich F., Law C.T., Lan G., Imaz M., Bowness J.S., Brockdorff N., Gaffney D, & Marioni J.C. (2021). Locus-specific expression of transposable elements in single cells with CELLO-seq. Nature biotechnology, 40(4), 546-554.
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