Erp72

HPW-RX40 (2-Methoxy-4-[(E)-2-nitrovinyl]phenyl 2,3-dichlorobenzoate) was synthesized according the methods described previously [17] (link). Bovine α-thrombin was purchased from Biovision, (Mountain View, CA, USA). Collagen (Type I, equine tendon) was from Helena Laboratories (Beaumont, TX, USA). Fibrinogen, U46619 (9,11-dideoxy-9,11-methanoepoxy PGF2α), 12-O-tetradecanoylphorbol-13-acetate (TPA), A23187, eosin isothiocyanate, oxidized glutathione (GSSG), and phenyl arsenoxide (PAO) were from Sigma-Aldrich (St. Louis, MO, USA). Alexa Flour 488-conjugated phalloidin, was obtained from Molecular Probes (Eugene, OR, USA). Thapsigargin and rutin were purchased from Cayman Chemical (Ann Arbor, MI, USA). Human recombinant PDI, ERp72, and Fura-2/AM were purchased from Enzo Life Sciences (Farmingdale, NY, USA); Human recombinant ERp57 and anti-P47 Ab were from Abcam (Cambridge, UK), and ERp5 was from ProSpec Protein Specialists (Rehovot, Israel). Anti-PDI Ab was from Pierce/Thermo (Rockford, IL, USA); anti-GRP78 Ab was from Genetex (Irvine, CA, USA); phospho-PKC substrate antibody and anti-CHOP Ab were from Cell Signaling Technology (Beverly, MA, USA); anti-actin was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All other chemicals were purchased from Sigma Chemical Co.