Mouse cxcl1 kc duoset elisa kit

Conditioned media from lung adenocarcinoma cells was collected as described above for C2C12 studies. Cytokine arrays were performed using Proteome Profiler Mouse XL Cytokine Arrays (R&D Systems) and visualized/quantified using a LI-COR Odyssey FC system. Ectopic cytokine studies were performed using C2C12 cells plated at a density of 100–500K/well of a 6-well tissue culture dish and differentiated as noted above for conditioned media experiments. All cytokines were purchased from Peprotech and used as noted in the text based on published IC50 ranges defined in other cell types/experimental contexts: IGFBP3 (cat#: 100-008), IGFBP6 (cat#: 350-07B), CXCL1 (cat#: 250-11), LIX/CXCL6 (cat#: 250-17), CCL2 (cat#: 250-10), CCL17 (cat#: 300-30), CCL20 (cat#: 250-27), and endostatin (cat#: 150-01). We noted substantial lot-to-lot variability with the CXCL1 preparations, so we advise recalibrating phenotypes if using different cytokine lots. CXCL1 ELISAs were performed using a Mouse CXCL1/KC DuoSet ELISA kit (R&D Systems) and serum or liquid nitrogen prepared whole muscle lysates from terminal tumor-bearing (531LN2) mice.