Trimethylolpropane trimethacrylate (TRIM), triethyleneglycol dimethacrylate, polyethylene glycol (10 kDa), 2-methoxyethanol, glycidylmethacrylate, Irgacure 184, N-Isopropylacrylamide (NIPAAm), ammonium persulfate (APS), tetramethyl-ethylenediamine (TEMED), 4-(2-acrylamidoethylcarbamoyl)-3-fluorophenylboronic acid (AmECFPBA) and methanol, N,N′-methylenebis- (acrylamide) (MBAAm) were purchased from Wako Pure Chemical Industries (Tokyo, Japan). Poly(vinyl alcohol) (Mw 13,000–23,000, 98% hydrolyzed) and fluorescein isothiocyanate (FITC)-labeled bovine insulin were purchased from Sigma-Aldrich (Tokyo, Japan). Mouse skin was purchased from Hoshino Laboratory Animals, Inc., Bandou-shi, Japan. Waterproof epoxy resin KE-3424-G was obtained from Shin-Etsu Chemical Co. Ltd., Tokyo, Japan.
Tetramethyl ethylenediamine temed
Manufactured by Fujifilm
Sourced in Japan
Tetramethyl-ethylenediamine (TEMED) is a chemical compound used as an accelerator in gel electrophoresis techniques, such as SDS-PAGE and native PAGE. It functions by catalyzing the polymerization of acrylamide and bisacrylamide to form the gel matrix.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium persulfate, by Fujifilm (1 mentions)
N isopropylacrylamide, by Fujifilm (1 mentions)
2 methoxyethanol, by Fujifilm (1 mentions)
N n methylenebis acrylamide mbaam, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Imidazole, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Dipotassium hydrogen phosphate, by Fujifilm (1 mentions)
Potassium dihydrogen phosphate, by Fujifilm (1 mentions)
2 protocols using tetramethyl ethylenediamine temed
1
Hydrogel Synthesis and Characterization
2
Purification and Characterization of Thermostable Phosphatase
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TL was provided by Amano Enzyme Inc. (Nagoya,
Japan). TL was dissolved in 100 mM sodium phosphate buffer, pH 6.0,
and purified from impurities using an Amicon ultrafiltration device
(50 kDa MWCO). Dipotassium hydrogen phosphate, sodium chloride, potassium
dihydrogen phosphate, para-nitrophenyl phosphate
(p-NPP), sodium dodecyl sulfate (SDS), hydrochloric
acid, tetramethylethylenediamine (TEMED), and sodium ampicillin were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Tryptone and yeast extract were purchased from Difco (Miami). Trizma
base and imidazole were purchased from Sigma Aldrich (Tokyo, Japan).
Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from
Takara Bio Inc. (Shiga, Japan). Acrylamide/bis solution 30% was purchased
from Nacalai Tesque, Inc. (Kyoto, Japan). All reagents were used as
received.
Japan). TL was dissolved in 100 mM sodium phosphate buffer, pH 6.0,
and purified from impurities using an Amicon ultrafiltration device
(50 kDa MWCO). Dipotassium hydrogen phosphate, sodium chloride, potassium
dihydrogen phosphate, para-nitrophenyl phosphate
(p-NPP), sodium dodecyl sulfate (SDS), hydrochloric
acid, tetramethylethylenediamine (TEMED), and sodium ampicillin were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Tryptone and yeast extract were purchased from Difco (Miami). Trizma
base and imidazole were purchased from Sigma Aldrich (Tokyo, Japan).
Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from
Takara Bio Inc. (Shiga, Japan). Acrylamide/bis solution 30% was purchased
from Nacalai Tesque, Inc. (Kyoto, Japan). All reagents were used as
received.
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