For the metabolic analyses, each sample consisted of 20-pooled fruits per plot. The extraction of the polyphenolic fraction was carried out according to the procedure reported by Choi et al. (2011) (link) with some changes. Briefly, frozen tomato powder (3 g) was weighed, placed into a 50 ml Falcon tube, and extracted with 15 ml of 70% methanol into an ultrasonic bath (Branson 5200, Ultrasonic, Corp.) for 30 min at 30°C. The mixture was centrifuged at 20000 g for 10 min at 4°C, and the supernatant was collected, while the pellet was re-extracted for the second time as previously described. An aliquot (500 μl) of the methanolic extract was stored at -20°C until further analyses, while 25 ml of extract were dried by rotary evaporator (Buchi R-210, Milan, Italy) at 30°C for 10 min and dissolved in 70% methanol (2 ml). Then, the extract was transferred in a glass tube and was further dried by using a SpeedVac (Thermo Scientific, Savant, SPD131DDA SpeedVac Concentrator, Waltham, MA, USA). The dried extract was dissolved in 70% methanol (500 μl) obtaining a final concentration of 5 g fresh weight (FW)/ml. The extract was passed through a 0.45 μm Millipore nylon filter (Merck Millipore, Bedford, MA, USA) and stored at -20°C until LC/MS/MS analysis.
Spd131dda speedvac concentrator
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SPD131DDA SpeedVac Concentrator is a laboratory equipment designed for concentrating liquid samples by evaporation. It uses a combination of vacuum and controlled temperature to efficiently remove solvents from samples, leaving behind the desired concentrate.
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Lab products found in correlation
4 protocols using spd131dda speedvac concentrator
1
Polyphenolic Profiling of Tomato Samples
2
Metabolite Extraction from Co-Culture
3
Extraction and Analysis of Pyochelin Compounds
4
Liposome Preparation by Dried Film Method
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Liposomes were created using the dried film method [9 (link)]. The DOPHT stored in chloroform was aliquoted into 4½-mL amber vials and dried to a film under a stream of argon and continually dried under vacuum overnight; DOPT stored in a chloroform/methanol 2:1 mixture was also dried to a film under argon and further dried under an overnight vacuum. Overnight vacuum was accomplished using a speed-vacuum condenser equipped with a Savant SPD131DDA SpeedVac concentrator and a RVT4104 refrigerated vapor trap (Thermo Fisher, Rockford, IL, USA). Dried lipid films were then hydrated in a buffer at the appropriate pH and periodically vortexed for nearly an hour. Hydrated lipids were then subjected to five cycles of freezing (ethanol on dry ice) and thawing (50 °C water bath) just prior to extrusion through double-stacked 100 nm pore filter paper housed in a LiposoFast hand-held extruder (Avestin, Inc.; Ottawa, ON, Canada). Liposomes were passed 11 times back and forth through the filters.
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