CoCl2 was purchased from Sigma-Aldrich (St. Louis, MO, USA). To establish a stable cell line, antibiotics (hygromycin and puromycin) were procured from Invitrogen (Waltham, MA, USA). The primary antibody against HIF-1α was obtained from BD Biosciences (San Diego, CA, USA). Antibodies against p-AMPK, AMPK, and α-enolase were purchased from Cell Signaling Technology (Beverly, MA, USA). All other antibodies, including those against actin, were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Antibodies against p ampk
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against p-AMPK are protein-based reagents that specifically recognize the phosphorylated form of the AMP-activated protein kinase (AMPK) enzyme. AMPK is a key regulator of cellular energy homeostasis, and its phosphorylation at specific sites is a critical indicator of AMPK activation. These antibodies can be used to detect and quantify the levels of phosphorylated AMPK in various biological samples, such as cell lysates or tissue extracts, using techniques like Western blotting or immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
P acc, by Cell Signaling Technology (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Hygromycin, by Thermo Fisher Scientific (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Primary antibody against hif 1α, by BD (1 mentions)
α enolase, by Cell Signaling Technology (1 mentions)
Cocl2, by Merck Group (1 mentions)
Sc 47778, by Santa Cruz Biotechnology (1 mentions)
Sc 133134, by Santa Cruz Biotechnology (1 mentions)
Alizarin red s st1078, by Beyotime (1 mentions)
Ab3242, by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
P creb, by Santa Cruz Biotechnology (1 mentions)
Pgc 1α, by Santa Cruz Biotechnology (1 mentions)
Srebp 1c, by Santa Cruz Biotechnology (1 mentions)
C ebpα, by Santa Cruz Biotechnology (1 mentions)
P hsl, by Santa Cruz Biotechnology (1 mentions)
Pparγ, by Santa Cruz Biotechnology (1 mentions)
4 protocols using antibodies against p ampk
1
Establishing HIF-1α Cell Line
2
Resveratrol and Dexamethasone Modulate Osteogenic Pathways
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Resveratrol (purity ≥99%; catalog number: R5010) and dexamethasone (≥98%; D1756) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Antibodies against p-AMPK (1:1000 dilution; 50081), AMPK (1:1000; 5832), p-ACC (1:1000; 11818), and ACC (1:1000; 3676) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β-Actin (1:1000; sc-47778), SOD1 (1:1000; sc-271014), and SOD2 (1:1000; sc-133134) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against PGC-1ɑ (1:500; AB3242) were from Millipore (Burlington, MA, USA). SIRT3 (1:1000; WL03840), Runx2 (1:1000; WL03358) and receptor activator of nuclear factor-κB ligand (RANKL; 1:600; WL00285) were from Wanleibio (Shenyang, China). MTT (ST1537) and Alizarin Red S (ST1078) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Minimum essential medium-alpha modification (α-MEM; 32571101), Dulbecco’s modified Eagle’s medium (DMEM; 10566016) and fetal bovine serum (10270–106) were purchased from Gibco (Carlsbad, CA, USA). TRIzol® Reagent (15596026) was obtained from Thermo Fisher Scientific (Waltham, MA, USA).
3
Comprehensive Protein Analysis in Adipocytes
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Antibodies against p-AMPK (#P-2535) and AMPK (#2532) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against UCP-1 (#Cat sc-293418), PGC-1α (#Cat sc-518038), p-CREB (#Cat sc-81486), CREB (#Cat sc-271), PPAR-γ (#Cat sc-271392), C/EBPα (#Cat sc-166258), p-HSL (#Cat sc-139656), HSL (#Cat sc-74489), FAS (#Cat sc-74540), SIRT-1 (#Cat sc-74540), SREBP-1c (#Cat sc-36553), and β-actin (#Cat sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Compound C (CC), H-89, and BAPTA-AM were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Nobiletin Modulates AMPK Signaling
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Nobiletin (purity ≥ 95%) was purchased from Wako Pure Chemicals (Osaka, Japan) (Figure 1 ). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), trypsin-ethylenediaminetetraacetic acid (EDTA), and penicillin-streptomycin were obtained from Gibco BRL (Grand Island, NY, USA). 6-[4-(2-Piperidin-1-ylethoxy)phenyl]-3-pyridin-4-ylpyrazolo[1,5-a]pyrimidine (Compound C), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), dimethyl sulfoxide (DMSO), lactate dehydrogenase (LDH) kit, and Oil red O (ORO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against p-AMPK, p-ACC, AMPK, and ACC were obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against SREBP-1c, FAS, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Enhanced chemiluminescence (ECL™) detection reagents were purchased from GE Healthcare (Buckinghamshire, UK). All other reagents and solvents were of analytical and HPLC grades.
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