Navios ex software

Manufactured by Beckman Coulter

Sourced in United States

The Navios EX Software is a data analysis software application designed for use with Beckman Coulter's Navios EX flow cytometry system. The software enables users to acquire, analyze, and manage flow cytometry data generated by the Navios EX instrument.

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Lab products found in correlation

4 protocols using navios ex software

Characterization of Mesenchymal Stem Cells by Flow Cytometry

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Cells were enumerated using Perfect-Count Microspheres™ (Cytognos, Salamanca, Spain) microbeads in a Navios EX flow cytometer (Beckman Coulter, Pasadena, CA, USA). Viability (%) was determined using the 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, Pasadena, CA, USA) exclusion method. Data were analyzed with Navios EX Software v2.0 (Beckman Coulter) software. In accordance with de International Society on Cell and Gene Therapy (ISCT) criteria [3 (link)], identity of MSC was evaluated by the expression of surface markers CD31 PacificBlue-conjugated (clone 5.6E; Beckman Coulter), CD45 KromeOrange-conjugated (clone J33; Beckman Coulter), CD73 PE-conjugated (clone AD-2; Beckman Coulter), CD90 FITC-conjugated (clone F15-42–1-5, Beckman Coulter), CD105 PC7-conjugated (clone TEA3/17.1.1; Beckman Coulter), and HLA-DR APC-conjugated (clone Immu-357; Beckman Coulter) in a Navios EX Device. Cells were stained for 15 min at room temperature, washed and resuspendend with DPBS. Acquisition was done using Navios EX, and raw data were analyzed with Navios EX Software (version 2.0, Beckman Coulter).

Torrents S., del Moral A.E., Codinach M., Rodríguez L., Querol S, & Vives J. (2023). Optimized reagents for immunopotency assays on mesenchymal stromal cells for clinical use. Immunologic Research, 71(5), 725-734.

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Multiparameter Flow Cytometry Analysis

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The cell phenotype was analyzed by flow cytometry using Navios Cytometer, Navios
EX software, version 2.0 and Kaluza software (Beckman Coulter, USA). The
following monoclonal antibodies (mAbs) and the corresponding isotypes were
purchased from Beckman Coulter (USA) specific for: cluster of differentiation
(CD)3, CD4, CD8, CD11c, CD14, CD40, CD80, CD86, CD123, Vγ9TCR, and major
histocompatility complex molecule – HLA-DR (human leukocyte antigen D-related).
The mAbs were conjugated with FITC (fluorescein isothiocyanate), Pacific Blue,
Phycoerythrin Cyanin (PC)7, Allophycocyanin (APC) Vio, APC-Alexa Fluor 750.

Anh B.V., Thao C.T., Cuong P.T., Thuy N.T., Diem H.H., Van Khanh B.T., Hue B.T., Uyen T.T., Tu N.D., Hoai T.T., Thanh N.L., Liem N.T, & Nhung H.T. (2020). Vγ9γδ T Cell Induction by Human Umbilical Cord Blood Monocytes-Derived, Interferon-α-Stimulated Dendritic Cells. Cancer Control : Journal of the Moffitt Cancer Center, 27(1), 1073274820974025.

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Characterization of Mesenchymal Stem Cells by Flow Cytometry

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Cell Viability and Yield Quantification

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Cells were enumerated at the end of harvesting processes and after final product concentration of 2D and 3D cultures by flow cytometry using Flow-Count Fluorospheres (Beckman Coulter, Brea, CA, USA) or Perfect-Count Microspheres (Cytognos, Salamanca, Spain) with a Navios EX device (Beckman Coulter). The percentage of cell viability was determined by 7-aminoactinomycin D (Beckman Coulter) staining, which binds specifically to DNA when the cell membrane is damaged by necrotic processes. Cells were incubated with 15 mL of 7-aminoactinomycin D for 5 min at RT in the dark and then washed with 3 mL of Dulbecco's Phosphate-Buffered Saline (DPBS, Lonza) by centrifugation at 340g, 5 min, RT, before cytometric acquisition. Acquired data were analyzed with Navios EX software (version 2.0; Beckman Coulter). Fold increase was calculated as the ratio of total harvested cell yield to initial cell number inoculated.

López-Fernández A., Codinach M., Coca M.I., Prat-Vidal C., Castaño J., Torrents S., Aran G., Rodríguez L., Querol S, & Vives J. (2024). Comparability exercise of critical quality attributes of clinical-grade human mesenchymal stromal cells from the Wharton's jelly: single-use stirred tank bioreactors versus planar culture systems. Cytotherapy, 26(5).

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