Antibodies against DYKDDDDK (FLAG) tag, p-Tyrosine (p-Tyr-100) were from Cell Signaling Technology (CST). Antibodies against β-actin and were from Sigma-Aldrich. Antibody against IDH1 was from R&D SYSTEMS. Antibodies against SHP2, PTPMT1 and PTP1B was from PROTEINTECH. Goat anti-Mouse IgG (H+L) secondary antibody and goat anti-rabbit IgG (H+L) secondary antibody were from Thermo Fisher Scientific. Antibodies against p-IDH1 Y42 and p-IDH1 Y391 were custom-made by SHANGHAI GENOMICS, INC. Anti-NOX2 antibody was purchased from Abcam and Bioss. p67phox was purchased from Santa Cruz. Anti-rabbit IgG (H+L) F(ab')2 Fragment (Alexa Fluor 488 conjugate) and Anti-mouse IgG (H+L) F(ab')2 Fragment (Alexa Fluor 555 conjugate) was purchase from Cell Signaling Technology.
Anti nox2 antibody
Manufactured by Abcam
Anti-NOX2 antibody is a laboratory reagent used to detect the presence and measure the levels of the NOX2 protein in biological samples. NOX2 is a subunit of the NADPH oxidase enzyme complex, which plays a role in the production of reactive oxygen species. This antibody can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of NOX2 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
P67phox, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions)
Antibody against idh1, by R&D Systems (1 mentions)
Goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions)
P47phox antibody, by Santa Cruz Biotechnology (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
3 protocols using anti nox2 antibody
1
Antibody Characterization for Immunoblotting
2
Western Blot for NOX2 Protein
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Cells transfected with siNOX2 or siCTRL were lysed, and proteins were extracted. Whole cell extracts were subjected to 10% SDS-PAGE. Resolved proteins were transferred to a nitrocellulose membrane, blocked, and then incubated overnight with the anti-NOX2 antibody (Abcam). Tubulin was used as a loading control (Sigma). Protein content was determined by densitometric scanning of immunoreactive bands, and intensity values were obtained through the densitometry of individual bands as compared with Tubulin and normalized against siCTRL.
3
Immunoprecipitation and Western Blot Analysis
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Immunoprecipitation was performed as described previously [24 (link)]. Briefly, cell lysates were immunoprecipitated with a p47phox antibody (Santa Cruz Biotechnology, Dallas, TX, USA) overnight at 4 °C. The immunocomplexes were collected by washing six times with lysis buffer, boiled in 1 × Laemmli sample buffer (Bio-Rad) for 10 min and subjected to Western blot analysis with an anti-Nox2 antibody (Abcam).
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