To analyze dark tea samples, a 7890A Agilent GC (Agilent Technologies, CA, USA) system equipped with a 5975C MSD detector (Agilent Technologies), autosampler (7683 B series, Agilent Technologies), and Chemstation software was used. An HP-5MS column (30 m×0.25 mm×0.25 µm film thickness) was used, and the gas carrier (helium at 99.999% of purity) was supplied at a constant rate of 1 mL/min. The injector temperature was 250°C, and the injection mode was splitless. The temperature program began at 50°C for 1 min and increased at a rate of 3°C/min until it reached 210°C (this temperature was held for 3 min), followed by temperature ramping at 15°C/min to a final temperature of 230°C. The mass spectrometer was operated under the electron impact (EI) mode at ionization energy of 70 eV. The aux temperature, MS source temperature, and MS quad temperature were set to 280°C, 230°C, and 150°C, respectively. A mass range of m/z 80–500 was scanned to confirm the retention times of target analytes and data were gathered in full scan mode. The solvent delay time was 2.8 min.
7683b series
Manufactured by Agilent Technologies
Sourced in United States
The 7683B series is a line of lab equipment manufactured by Agilent Technologies. The 7683B series is designed for automated sample injection and extraction, providing consistent and reliable performance for a variety of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
7890n series, by Agilent Technologies (2 mentions)
5975c inert xl msd series, by Agilent Technologies (2 mentions)
Hp 1 fused silica capillary column, by Agilent Technologies (2 mentions)
Hp 7890a, by Agilent Technologies (2 mentions)
Agilent 5975c, by Agilent Technologies (2 mentions)
Agilent 7890a, by Agilent Technologies (2 mentions)
Agilent 7890a gc, by Agilent Technologies (1 mentions)
5975c series, by Agilent Technologies (1 mentions)
Nylon syringe filter, by Cytiva (1 mentions)
Db 5ms column, by Agilent Technologies (1 mentions)
Methanol, by Merck Group (1 mentions)
Gc 7890a, by Agilent Technologies (1 mentions)
Combi pal, by CTC Analytics (1 mentions)
Whatman, by Cytiva (1 mentions)
5975c msd, by Agilent Technologies (1 mentions)
Db 5ms, by Agilent Technologies (1 mentions)
Hp 5ms capillary column, by Agilent Technologies (1 mentions)
6890 gc ms, by Agilent Technologies (1 mentions)
6890n network gas chromatographic system, by Agilent Technologies (1 mentions)
12 protocols using 7683b series
1
GC-MS Analysis of Dark Tea Samples
2
Chemical Analysis of Organic Solvents in PR Products
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Organic solvents contained in PR products were analyzed after dilution with carbon disulfide (CS2; Kanto, Japan) and methanol (99.8%; Sigma Aldrich, USA). Qualitative analysis was first performed to identify organic solvents in 51 PR products, and further analysis was conducted for quantitation of the identified 20 chemicals. Diluted samples were sonicated for 30 min at room temperature, and viscous chemical samples were filtered through a nylon syringe filter (13 mm, 0.2 μm, Whatman, USA). Qualitative analysis was conducted by gas chromatography (GC, 7890A; Agilent Technology, USA)–mass spectrometry (MS, 5975C Series; Agilent Technology, USA) and auto sampler (Combi PAL, CTC analytics, Switzerland) in scan mode. A DB-5MS column (122-5532; Agilent Technology, USA) was used for analysis. Each mass spectrum was matched up with a GC-MS library (W10N11), and the chemical matching rate selected was higher than 80%.
Then, quantitative analysis was conducted by GC (6890N; Agilent Technology) with a flame ionization detector (FID) and auto sampler (7683B Series; Agilent Technology). The chemical used for quantitative analysis was selected from chemicals detected from the qualitative analysis listed in the MSDS, or if not listed in the MSDS, a chemical known to be toxic was selected. An EN-5 column (053139; SGE Analytical Science, Australia) was used for the analysis.
Then, quantitative analysis was conducted by GC (6890N; Agilent Technology) with a flame ionization detector (FID) and auto sampler (7683B Series; Agilent Technology). The chemical used for quantitative analysis was selected from chemicals detected from the qualitative analysis listed in the MSDS, or if not listed in the MSDS, a chemical known to be toxic was selected. An EN-5 column (053139; SGE Analytical Science, Australia) was used for the analysis.
3
Extraction and Quantification of Oil Components
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Extraction of oil components with dichloromethane (DCM) was done as described by Agrawal et al. (2012) (link). Prior to DCM extraction of the oil, 50 μL of squalene and mesitylene were added to the 1 mL oil layer of each incubation as internal standards for quantifying the depletion of n-alkanes and alkylbenzenes, respectively. Following this, 9 mL of DCM was added to extract the oil components and one microliter (μL) of the oil-DCM layer was injected by an autoinjector (7683B series, Agilent Technologies) into a GC (7890N series, Agilent Technologies), connected to an MS (5975C inert XL MSD series, Agilent). The GC was equipped with an HP-1 fused silica capillary column (length 50 m, inner diameter 0.32 mm, film thickness 0.52 μm; J&W Scientific) with helium as carrier gas. The amounts of oil components utilized for the reduction of the electron acceptors were determined as the decrease in the ratio of the peak area for a given component to that of the internal standard.
4
GC-MS Analysis of Propolis Extracts
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GC-MS analysis of propolis extracts was carried out using a GC (HP-7890A, Agilent technologies, USA) directly coupled with spectrometer (MSD-5975C, Agilent technologies, USA). Chromatographic separations was achieved by an HP5-MSI capillary column, 30 m in length × 0.25 mm in diameter, 0.25 µm film thickness (J & W Scientific, Folsom, USA) immobilized with 5% phenyl methyl silicone as the stationary phase. An aliquot (2 µl) of diluted propolis sample was injected into the GC-MS instrument, in the splitless mode, using an auto sampler (7683B series, Agilent technologies, USA) at an injector temperature of 270°C.The sample was then transported by Helium gas (99.99% purity) as the carrier gas at a flow rate of 1.2 ml min−1. The oven temperature was programmed at 35°C where it was held for 5 min followed by a gradual increase at the rate of 10°C min−1 to 280°C where it was held at an isothermal state for 10 min. The transfer line temperature was at 280°C while the temperature of the ion source was 230°C and the ionization energy was 70 eV. Fragment ions analysed in scan mode over 40–450 m/z mass range and MS quadrupole temperature was maintained at 150°C.
5
GC-MS Analysis of Derivatized Samples
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Agilent GC-MS system comprising of Agilent 7890A gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled with an Agilent 5975C mass detector (Agilent Technologies) was used. Derivatized sample (1 μL) was injected into GC-MS by automatic sampler (7683 B series, Agilent Technologies) with a split ratio of 1:10. Samples were separated on a fused silica capillary column DB-5ms ((5%-phenyl)-methylpolysiloxane: 30 m length, 0.25 mm internal diameter, 0.25 µm film, Agilent Technologies). The temperature program was as follows: initial temperature of 70 °C for 5 min, followed by final temperature increase to 300 °C at the ramp rate of 10 °C/min, and finally held at 300 °C for 10 min. Total run time calculated was 38 min. The inlet temperature and interface temperature were set at 280 °C. Each sample was replicated three times. Scan was started after solvent delay of 5 min with scan frequency of 4 s−1 (2.0 HZ).
6
Quantitative Analysis of Alkylbenzenes in Oils
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Oils (1 ml) were diluted with 9 ml of DCM. A 1 μl volume of the DCM extract was injected with an autoinjector (7683B series, Agilent Technologies, Santa Clara, CA, United States) into a gas chromatograph (7890N series, Agilent) equipped with an HP-1 fused silica capillary column (length 50 m, inner diameter 0.32 mm, film thickness 0.52 μm; J&W Scientific) with helium as the carrier gas and connected to a mass-selective detector (5975C inert XL MSD series, Agilent). Duplicate measurements were done on all the oil samples. Concentrations of toluene, ethylbenzene, o-xylene, and m/p-xylene in the oils were calculated based on the standard line obtained from the peak areas of pure individual standard alkylbenzenes (Sigma–Aldrich) analyzed by GC-MS.
7
GC-MS Analysis of Propolis Samples
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Based on the phytochemical contents and level of bioactivity, representative samples from hot and dry climatic conditions and cooler climatic conditions were chosen for GC-MS analysis. This was done using a gas chromatograph (HP-7890A, Agilent Technologies, USA) coupled with a mass spectrometer (MS-597C, Agilent Technologies, USA). The samples were extracted using absolute ethanol, and the supernatant evaporated to complete dryness leaving a solid residue. The residue was redissolved in dichloromethane to make one part per million and subjected to the GC-MS analysis. Chromatographic separations were achieved by a HP-5MS capillary column, 30 × 0.25 mm i.d., 0.25 µm thick (J & W Scientific, USA) immobilized with 5% (phenylmethyl silicone) as the stationary phase.
In the splitless mode, 1 µl of the propolis sample was injected into the GC-MS instrument using an autosampler (7683B series, Agilent Technologies, USA). The sample was then transported by helium (99.99% purity) as the carrier gas at a flow rate of 1.2 ml min−1. First, the oven temperature was programmed at 35°C, where it was held for 5 min, followed by a gradual increase at the rate of 10°C min−1 to 280°C, where it was held at an isothermal state for 30 min.
In the splitless mode, 1 µl of the propolis sample was injected into the GC-MS instrument using an autosampler (7683B series, Agilent Technologies, USA). The sample was then transported by helium (99.99% purity) as the carrier gas at a flow rate of 1.2 ml min−1. First, the oven temperature was programmed at 35°C, where it was held for 5 min, followed by a gradual increase at the rate of 10°C min−1 to 280°C, where it was held at an isothermal state for 30 min.
8
GC-MS Quantification of Organic Compounds
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Two mg of each sample were dissolved in 1 mL of methanol containing 0.025 μg·mL−1 of codeine, used as the internal standard, and injected into a gas chromatograph (Agilent Technologies 6890N, Agilent Technologies, Santa Clara, CA, USA) coupled with mass spectrometry (Agilent Technologies 5975, Agilent Technologies, Santa Clara, CA, USA), both of which were obtained from Hewlett Packard, Palo Alto, CA, USA. The equipment was operated using electronic impact ionization (EI) at 70 eV with an autoinjector, 7683B Series (Agilent Technologies, Santa Clara, CA, USA). The column was a Tecknokroma TR-45232 Sapiens-X5MS (30 m × 0.25 mm, film thickness 0.25 µm), and the splitless injection volume was 1 µL. The temperature gradient was as follows: 12 min at 100 °C, 100–180 °C at 15 °C/min, 1 min of retention at 180 °C, 180–300 °C at 5 °C/min, and 10 min of holding at 300 °C. The injector and detector temperatures were 250 and 280 °C, respectively. The carrier gas (He) flow rate was 1 mL/min.
9
GC-MS Analysis of Alkaloids
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The alkaloids were analyzed by a GC–MS carried out on the “Agilent Technologies 6890N” gas chromatographer, coupled with the “Agilent Technologies 5975 inert” mass detector operating on EI mode, and using the autoinjector “7683B Series Agilent Technologies”. The oven ramp temperature used was the following: 55–60 °C at 2.5 °C/min, 60–100 °C at 40 °C/min, 100–180 °C at 8 °C/min, and 180–300 °C at 6 °C/min. The injector temperature was 250 °C, operating on the Pulsed Spitless mode. The capillary column used was TEKNOKROMA TR-45232 Sapiens-X5MS (30 m × 250 µm ID × 0.25 µm). The interphase detector temperature was 280 °C, and the injection volume was of 1 µL.
10
GC-MS Analysis of Organic Compounds
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Samples were injected in split mode (2 μL, split ratio 20:1) at an inlet temperature of 220 °C on a Hewlett Packard 6890 GC-MS equipped with a 5973 mass selective detector (MSD), and an Agilent 7683B series injector and autosampler. Separation was performed on a Zebron ZB5-HT-INFERNO column (5% phenyl methyl siloxane; length: 35 m; diameter: 250 μm) with guard column. Helium was used as mobile phase at a constant flow rate of 1.2 mL/min and average velocity 37 cm/s. After 5 min at 80 °C, the column temperature was increased to 110 °C at a rate of 2.5 K/min, then to 280 °C at 120 K/min, and kept at 280 °C for another 4 min. A solvent delay of 5 min was allowed before collecting MS spectra at a fragmentation energy of 70 eV.
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