Fluorochrome-conjugated anti-mouse monoclonal antibodies (Abs) specific for CD8-α, CD274, CD279, tumor necrosis factor (TNF), interferon-γ (IFN-γ), and interleukin-2 (IL-2) were purchased from eBiosciences (San Diego, CA). Anti-PD-1 and anti-PD-L1 monoclonal antibodies used for immunofluorescence were purchased from Abcam (Cambridge, MA). For depletion studies, the 2.43 hybridoma was purchased from ATCC (Manassas, VA). The anti-PD-L1 monoclonal antibody (clone 10F.9G2) used for in vivo blockade experiments was purchased from BioXCell (West Lebanon, NH). K7M2 osteosarcoma cells were purchased from ATCC.
Fluorochrome conjugated anti mouse monoclonal antibodies abs
Manufactured by Thermo Fisher Scientific
Fluorochrome-conjugated anti-mouse monoclonal antibodies (Abs) are laboratory reagents used in flow cytometry and other immunoassay techniques. These antibodies are specific to mouse antigens and are labeled with fluorescent dyes, allowing for the detection and quantification of target molecules in cell samples.
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2 protocols using fluorochrome conjugated anti mouse monoclonal antibodies abs
1
Tumor Immunology Protocol
2
Immune Cell Profiling in Glioblastoma
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Fluorochrome-conjugated anti-mouse monoclonal antibodies (Abs) specific for CD8α, CD274, CD279, CTLA-4, CD86, tumor necrosis factor (TNF), interferon gamma (IFNγ), interleukin-2 (IL-2), CD4, FoxP3, NKp46, CD3, and interleukin-10 (IL-10) were purchased from eBiosciences (San Diego, CA) and diluted 1:200 prior to use. Anti-CD8 depletion antibodies were purified from the mouse 2.43 hybridoma cell line purchased from ATCC (Manassas, VA). Bioluminescent GL261-Luc 2 cells were derived and grown as previously described [6 (link)].
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