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Ab9050 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a tool designed for research and analytical purposes. The core function of Ab9050 is to facilitate scientific investigations, but no further details about its intended use or capabilities can be provided in an unbiased and factual manner.

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2 protocols using ab9050

1

Chromatin Immunoprecipitation of Histone Modifications in Ventral Tegmental Area

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For each ChIP sample for histone modifications, VTA punches (2 punches) were collected from one control or chronic morphine-treated rat (14 days of morphine administration followed by 14 days of withdrawal) (n = 8 -11 for each ChIP). For each ChIP sample for key histone modifying enzymes and related regulatory proteins, VTA punches were pooled from two rats (4 punches) (n = 14 – 22). Anti-acH3 (rabbit polyclonal, Millipore, 06-599), anti-acH4 (rabbit polyclonal, Millipore, 06-598), anti-H3K4me3 (rabbit monoclonal, Millipore, 17-614), anti-H3K9me2 (mouse monoclonal, Abcam, ab1220), anti-H3K9me3 (rabbit polyclonal, Abcam, ab8898), anti-H3K27me3 (mouse monoclonal, Abcam, ab6002), anti-H3K36me3 (rabbit polyclonal, Abcam, ab9050), anti-mSIN3a (rabbit polyclonal, Santa Cruz, sc-994X), anti-ING2 (rabbit polyclonal, Santa Cruz, sc-134973), anti-KMT2A/MLL (rabbit polyclonal, Millipore, ABE240), anti-KMT1C/G9a (rabbit polyclonal, Abcam, ab40542), anti-SUZ12 (rabbit monoclonal, Cell Signaling, 3737S), anti-EZH2, anti-RING1A (rabbit polyclonal, Abcam, ab32644), anti-RING1B (rabbit monoclonal, Cell Signaling, 5694S), and anti-BMI1 (rabbit monoclonal, Cell Signaling, 6964S) were used.
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2

Chromatin Immunoprecipitation of Histone Modifications in Ventral Tegmental Area

Check if the same lab product or an alternative is used in the 5 most similar protocols
For each ChIP sample for histone modifications, VTA punches (2 punches) were collected from one control or chronic morphine-treated rat (14 days of morphine administration followed by 14 days of withdrawal) (n = 8 -11 for each ChIP). For each ChIP sample for key histone modifying enzymes and related regulatory proteins, VTA punches were pooled from two rats (4 punches) (n = 14 – 22). Anti-acH3 (rabbit polyclonal, Millipore, 06-599), anti-acH4 (rabbit polyclonal, Millipore, 06-598), anti-H3K4me3 (rabbit monoclonal, Millipore, 17-614), anti-H3K9me2 (mouse monoclonal, Abcam, ab1220), anti-H3K9me3 (rabbit polyclonal, Abcam, ab8898), anti-H3K27me3 (mouse monoclonal, Abcam, ab6002), anti-H3K36me3 (rabbit polyclonal, Abcam, ab9050), anti-mSIN3a (rabbit polyclonal, Santa Cruz, sc-994X), anti-ING2 (rabbit polyclonal, Santa Cruz, sc-134973), anti-KMT2A/MLL (rabbit polyclonal, Millipore, ABE240), anti-KMT1C/G9a (rabbit polyclonal, Abcam, ab40542), anti-SUZ12 (rabbit monoclonal, Cell Signaling, 3737S), anti-EZH2, anti-RING1A (rabbit polyclonal, Abcam, ab32644), anti-RING1B (rabbit monoclonal, Cell Signaling, 5694S), and anti-BMI1 (rabbit monoclonal, Cell Signaling, 6964S) were used.
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