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Sc 2044

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Sc-2044 is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a tool for researchers and scientists in their work.

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Lab products found in correlation

A11055, by Thermo Fisher Scientific (1 mentions) D1306, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated donkey anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa 555 conjugated donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) A21432, by Thermo Fisher Scientific (1 mentions) Fluoview fv1000, by Olympus (1 mentions) Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) L1516 2mg, by Merck Group (1 mentions) Alexa fluor 647 donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Mowiol 4 88 reagent, by Merck Group (1 mentions)

3 protocols using sc 2044

1

Immunochemistry and Immunofluorescence Protocol for Brain Sections and Cultured Neurons

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Immunochemistry and immunofluorescence were performed as described previously.63 (link),64 (link) Frozen 20-μm thick brain sections or cultured neurons placed on Confocal dish (Corning, CLS-DL-CC-014) were fixed in 4% paraformaldehyde, blocked by 8% normal donkey serum (Santa Cruz Biotechnology, sc-2044), and incubated in specific primary antibodies as follows: goat anti-LC3 (1:500), mouse anti-TUBB3 (1:1000), rabbit anti-cleaved CASP3 (1:200), mouse anti-ARRB1 (1:200) and rabbit anti-BECN1 (1:500). After being washed 3 times by PBST (0.1% Tween 20 in PBS [70011-044, Gibco]), the sections and cells were incubated with corresponding Alexa 488-conjugated donkey anti-mouse (Molecular Probes, A-21202), Alexa 555-conjugated donkey anti-rabbit (Molecular Probes, A-21432) and Alexa 647-conjugated donkey anti-goat (Molecular Probes, A11055) secondary antibodies. DAPI (Molecular Probes, D1306) was used to stain nuclei.65 (link) The immunofluorescence TUNEL assay was performed according to the instructions of the manufacturer.8 (link),55 (link) Images were obtained by confocal microscopy (Olympus, Fluoview FV1000, Tokyo, Japan).
Wang P., Xu T.Y., Wei K., Guan Y.F., Wang X., Xu H., Su D.F., Pei G, & Miao C.Y. (2014). ARRB1/β-arrestin-1 mediates neuroprotection through coordination of BECN1-dependent autophagy in cerebral ischemia. Autophagy, 10(9), 1535-1548.
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2

Immunohistochemical Analysis of Ovarian Markers

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Dissected ovaries were cultured overnight and subsequently treated with γ-irradiation
as indicated. Ovaries were fixed in formalin, embedded in paraffin and sectioned into
6 µm thickness (Morphisto GmbH, Frankfurt, Germany). For 3,3'-Diaminobenzidine (DAB)
IHC staining sections were deparaffinised and rehydrated followed by 30 min antigen
retrival in boiling 0.1 M citrate buffer. Sections were blocked for 1 hr at room
temperature in 5% donkey normal serum (Santa Cruz, sc-2044) in TBS and incubated with
primary antibody raised either against the oocyte marker Msy (Santa Cruz, N-13,
1:200), p53 (Santa Cruz, DO-1, 1:100), p63 (Santa Cruz, H-129, 1:200) or p73 (Merck
Millipore, ER-15, 1:100) in 1% BSA in TBS overnight. Sections were developed after
incubation with biotin-conjugated secondary antibodies for 1 hr at room temperature
in 1% BSA in TBS (Vector Labs) with the ABC DAB Peroxidase System (Vector Labs).
Nuclei were stained for 5 min in Mayer’s hematoxylin followed by dehydration and
mounting of the stained sections.
Coutandin D., Osterburg C., Srivastav R.K., Sumyk M., Kehrloesser S., Gebel J., Tuppi M., Hannewald J., Schäfer B., Salah E., Mathea S., Müller-Kuller U., Doutch J., Grez M., Knapp S, & Dötsch V. (2016). Quality control in oocytes by p63 is based on a spring-loaded activation mechanism on the molecular and cellular level. eLife, 5, e13909.
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Immunohistochemical Labeling Procedure

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Free-floating sections were rinsed 7 times for 15 min each with 1x tris-PBS (TPBS; Tris–HCl 10 mM, sodium phosphate buffer 10 mM, 0.9% NaCl, pH 7.4), and incubated with the following primary antibodies: (1) rabbit polyclonal anti-c-Fos antibody (RPCA-c-Fos-AP, Encorbio, USA), diluted 1:500; (2) Wisteria floribunda agglutinin (WFA; L1516–2MG, Sigma Aldrich, USA), diluted 1:200; (3) mouse monoclonal anti-PV antibody (235, Swan, Swiss antibodies, Switzerland), diluted 1:1000; or (5) mouse monoclonal anti-CB antibody (300, Swan, Swiss antibodies, Switzerland) diluted 1:1500; (6) mouse monoclonal anti-CaMKII antibody (MA 1–048, Thermo Scientific, USA) diluted 1:50. Incubations were at 4º C for 48 hr in TPBS 0.1M Triton X-100 containing 3% of donkey serum (Santa Cruz Biotechnology sc-2044). After three 15-min TPBS rinses, tissue was incubated for 2 hr at room temperature protected from light with one of the following secondary antibodies with conjugated fluorochromes: (1) Alexa Fluor 488 donkey anti-rabbit (A-21206, Life Technologies, USA), diluted 1:500; (2) Alexa Fluor 647 donkey anti-mouse (715–605-150, Jackson Labs, USA), diluted 1:500; or (3) biotinylated goat anti-rabbit conjugated with streptavidin Texas red (Vector Labs, UK), diluted 1:500. Once the fluorescence reaction occurred, sections were mounted using Mowiol 4–88 reagent (475904–100GM, EMD Millipore, USA).
Vazquez-Sanroman D., Arlington W.G, & Bardo M.T. (2020). Effects of social isolation on perineuronal nets in the amygdala following a reward omission task in female rats. Molecular neurobiology, 58(1), 348-361.
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