Anti vimentin

Manufactured by Cell Marque

Sourced in United States

Anti-vimentin is a laboratory reagent used in immunohistochemistry and immunocytochemistry procedures. It is a monoclonal antibody that specifically binds to the vimentin protein, which is a type III intermediate filament found in various cell types.

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Lab products found in correlation

Anti c5b 9, by Agilent Technologies (1 mentions) Mac387, by Abcam (1 mentions) Anti calprotectin, by Abcam (1 mentions) Anti c3c, by Agilent Technologies (1 mentions) Anti α sma, by Merck Group (1 mentions) Anti α smooth muscle actin α sma, by Merck Group (1 mentions)

Close spelling variants of this product

Vimentin

2 protocols using anti vimentin

Kidney Biopsy Analysis Protocol

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Immunofluorescence: biopsy samples of kidney cortex were processed for cryosectioning. Antibodies: Anti Factor B (Novus Biologicals, UK), anti C4d, anti C1q (AbD Serotec, France), anti C3c, anti C5b-9 (DAKO, France), anti MBL, anti MASP2 (Cliniscience, France). Each slide was semi quantitatively evaluated by a trained nephropathologist.
Immunohistochemistry: biopsies were preserved in formalin. Antibodies: anti-vimentin (Cell-Marque, USA), anti-αSMA (alpha smooth muscle actin); (Sigma, St. Louis, MO), anti-Calprotectin reacting with Monocyte/Granulocytes/Macrophages (MAC-387, AbCam, France) and anti-CD3 (SouthernBiotech, Birmingham, Alabama, USA). Fibrosis was analyzed by Sirius Red staining. Staining quantification was performed in silico (Visilog 6.9 software): fibrosis: percent of stained area per field; inflammation: number of positive immune cells per field. We evaluated 10 fields (×100) per tissue sample.

Delpech P.O., Thuillier R., SaintYves T., Danion J., Le Pape S., van Amersfoort E.S., Oortwijn B., Blancho G, & Hauet T. (2016). Inhibition of complement improves graft outcome in a pig model of kidney autotransplantation. Journal of Translational Medicine, 14, 277.

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Quantifying Kidney Graft Remodeling

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Biopsy samples from corticomedullary kidney collected at day 14 or 3 months after transplantation were fixed with 4% formalin and paraffin-embedded. For immunohistochemistry, we used the following antibodies: anti-vimentin (Cell-Marque, USA), anti α-smooth muscle actin (α-SMA, Sigma-aldrich, France) and anti-SWC3a reacting with monocytes/macrophages (553640; BD-Pharmingen, France), positive staining was revealed using Diaminobenzidine colorimetric reaction (DAKO, France). Vimentin staining quantification was performed by counting the number of vimentin positive tubes per field ; α-SMA and Picro-Sirius staining quantification was performed by quantifying the percentage of staining per field (Visilog 6.9 software); and evaluation for SWC3a staining was performed by counting the number of positive cells per field. We evaluated 10 fields per tissue sample (magnification x100 to Picro-Sirius, -SMA and Vimentin, and x200 to leukocytes infiltrate and SWC3a).

Tillet S., Giraud S., Kerforne T., Saint-Yves T., Joffrion S., Goujon J.M., Cau J., Mauco G., Petitou M, & Hauet T. (2016). Inhibition of coagulation proteases Xa and IIa decreases ischemia-reperfusion injuries in a preclinical renal transplantation model. Translational research : the journal of laboratory and clinical medicine, 178.

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