Glucose oxidase, Cyclosporine A, Sanglifehrin A, DCFH-DA, DAPI, calcein-AM and Rhodamine-123 were purchased from the Sigma Chemical Corp (St. Louis, USA). The molecular probes including MitoSOX™, Mito-Tracker-Red/Green were obtained from Invitrogen Corporation. (Carlsbad, USA). The Malondialdehyde assay kits were purchased from Beyotime Institute of Biotechnology (Nanjing, CHINA). The Annexin V-FITC/Propidium iodide apoptosis kit, TUNEL apoptosis detection kit, Mitochondria Fractionation Kit, and Nucleus Fractionation Kit was purchased from the Roche (Cambridge, USA). GAPDH, COX IV, Bax, Bcl-2, cytochrome C antibodies, Pifithrin-α, Pifithrin-μ and ionomycin were obtained from Santa Cruz Biotechnology (Santa Cruz, USA). P53 antibodies, Goat anti-Rabbit IgG-FITC, and Goat anti-Rabbit IgG-(H+L)-HRP were purchased from the abcam (Cambridge, USA). Caspase-9 and Caspase-3 Colorimetric Assay Kit was obtained from Keygen (Nanjing, China). All other chemicals and solvents were of analytical grade with highest purity commercially available.
Malondialdehyde assay kit
Manufactured by Beyotime
Sourced in China
The Malondialdehyde Assay Kit is a colorimetric assay used for the quantitative determination of malondialdehyde (MDA) levels in various biological samples. MDA is a naturally occurring compound that serves as a marker for oxidative stress and lipid peroxidation.
Automatically generated - may contain errors
Lab products found in correlation
Superoxide dismutase, by Beyotime (2 mentions)
Catalase assay kit, by Beyotime (2 mentions)
Ionomycin, by Santa Cruz Biotechnology (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Goat anti rabbit igg h l hrp, by Abcam (1 mentions)
Goat anti rabbit igg fitc, by Abcam (1 mentions)
Pifithrin α, by Santa Cruz Biotechnology (1 mentions)
Cox 4, by Santa Cruz Biotechnology (1 mentions)
Cytochrome c antibody, by Santa Cruz Biotechnology (1 mentions)
Pifithrin μ, by Santa Cruz Biotechnology (1 mentions)
Sanglifehrin a, by Merck Group (1 mentions)
Cyclosporine a, by Merck Group (1 mentions)
Calcein am, by Merck Group (1 mentions)
Dcfh da, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Glucose oxidase, by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
Tunel apoptosis detection kit, by Roche (1 mentions)
9 protocols using malondialdehyde assay kit
1
Apoptosis and Oxidative Stress Assays
2
Nasal Tissue Protein Analysis
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Nasal tissue proteins were extracted with a protein extraction kit (Beyotime, Shanghai, China). The levels of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were measured by superoxide dismutase, glutathione peroxidase and malondialdehyde assay kits according to manufacturer's instructions (Beyotime). Total protein concentration was determined by a bicinchoninic acid protein assay kit (Beyotime), and enzyme activity was standardized to milligram protein.
3
Neural stem cell culture protocol
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PQ was purchased from Sigma Chemical Co. (Sigma-Aldrich, Milan, Italy). ReNcell NSC Maintenance Medium and accutase were obtained commercially from Millipore (Temecula, CA). Epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2) were purchased from PeproTech. Laminin was purchased from Invitrogen (Carlsbad, CA, USA). Catalase Assay Kit, Malondialdehyde Assay Kit, Lactate Dehydrogenase Assay Kit, BCA Protein Assay Kit, Cell Lysis Buffer for Western and IP, goat anti-rabbit IgG-HRP, and goat anti-mouse IgG-HRP were obtained from Beyotime (Jiangsu, China). Total Superoxide Dismutase Assay Kit was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). Tripure was obtained from Roche (Basel, Switzerland). The AMV first strand cDNA Synthesis Kit was purchased from MBI (Fermentas, Canada). Real-time PCR Kit was obtained from Tiangen Biotech (Beijing, China). Rabbit anti-Nrf2 polyclonal antibody, rabbit anti-Keap1 polyclonal antibody, rabbit anti-PKC polyclonal antibody, and rabbit anti-CKII polyclonal antibody were purchased from GeneTex (San Antonio, USA). Mouse anti-β-tubulin polyclonal antibody was purchased from Boster (Wuhan, China).
4
Quantifying Malondialdehyde in Pitaya Peel
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Malondialdehyde (MDA) content in pitaya peel was determined according to the Malondialdehyde Assay Kit (Beyotime, Shanghai, China). MDA content was expressed as μmol kg−1 of fresh fruit weight.
5
Oxidative Stress Biomarker Quantification
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Above 30 μL supernatants were collected to estimate the levels of catalase (CAT), malondialdehyde (MDA), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). The levels of CAT (CAT Assay Kit, R&D Systems Ltd., MI, USA), MDA (malondialdehyde assay kit, Beyotime Institute of Biotechnology, Beijing, China), SOD (SOD determination kit, Fluka, St. Louis, MO, USA), and GSH-Px (GSH-Px assay kit, Nanjing Jiancheng Biology Research Institute, Nanjing, China) were measured by using corresponding kits.
6
Enzymatic Activities and Oxidative Stress
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Cells (1×104/well) were seeded into a 96-well plate and incubated at 37°C for 24 h. Then, the cells were centrifuged at 200 g for 5 min at 4°C. After being washed with PBS, the cells were resuspended and collected for the detection of the enzymes activities. The cellular SOD and CAT enzyme were determined using corresponding kits (Beijing Solarbio Science & Technology Co., Ltd.), according to the protocols. The MDA content was detected with thiobarbituric acid substance provided by Malondialdehyde Assay Kit (Beyotime, China).
7
PI3K/Akt Pathway Modulation in Apoptosis
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3% H2O2 solution and dimethyl sulfoxide were obtained from Sigma (St. Louis, MO). RPMI-1640 culture medium, 0.05%Trypsin-EDTA, and fetal bovine serum (FBS) were from Gibco (Grand Island, NY, USA). One step TUNEL apoptosis assay kit, puromycin dihydrochloride, bicinchoninic acid assay kit, glutathione peroxidase kit, catalase assay kit, DAPI staining solution, DAF-FM diacetate kit, dihydroethidium, superoxide dismutase, and malondialdehyde assay kit were obtained from Beyotime (Shanghai, China). Anti-Bax, anti-Bcl-2, and anti-Caspase-3 were from Abcam (Cambridge, MA, USA). Anti-PI3K, anti-Akt, antiphosphorylation-PI3K, and antiphosphorylation-Akt were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Immobilon-PSQ transfer membrane was obtained from Millipore (Billerica, MA, USA). An Annexin V/FITC apoptosis detection kit was from BD Biosciences (San Diego, CA, USA). PI3-kinase LY 294002 was purchased from MedChemExpress LLC (New Jersey, USA). Lipofectamine™ 3000 transfection reagent was purchased from Thermo Fisher Scientific, Inc. (Invitrogen, USA). Lentivirus control and PI3K shRNA (U6-MCS-Ubiquitin Cherry-IRES-puromycin) were purchased from GeneChem (Shanghai, China).
8
Malondialdehyde Quantification Protocol
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Measurement of MDA content was performed by the Malondialdehyde Assay Kit (Beyotime, Shanghai, China). MDA content was expressed on a fresh weight basis as μmol/mg.
9
Cardiac Antioxidant Evaluation Protocol
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Proteins of the heart were collected according to the guidelines of manufacturer (Nanjing Jiancheng Chemical Co, Nanjing, China) and the concentrations were determined by BCA assay (Pierce). Total antioxidant capacity (T-AOC) and SOD activity from tissue were determined by T-AOC (according to the redox potential of Fe3 + -TPTZ) detection kit and SOD activity (WST-1 method) assay kit (Nanjing Jiancheng Chemical Co), respectively according to the manufacturer's instructions. Enzyme activities were normalized to protein concentration in samples (U/mg protein). Intracellular total glutathione (GSH) was measured using a Total Glutathione Assay Kit (Beyotime, Nantong, China) following the manufacturer's protocol. Malondialdehyde (MDA), a lipid peroxidation product, was measured using the Malondialdehyde Assay Kit (Beyotime) by thiobarbituric acid assay, which is based on thiobarbituric acid (TBA) reacted with MDA to produce thiobarbituric acid reactive substances. The content of GSH and MDA was normalized to protein concentration and expressed as μmol/mg protein and nmol/mg protein, respectively.
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