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Hplc grade ammonium formate

Manufactured by Thermo Fisher Scientific
Sourced in India, United States

HPLC grade ammonium formate is a high-purity chemical used as a buffer and mobile phase additive in high-performance liquid chromatography (HPLC) applications. It is a crystalline salt with the chemical formula CH3COO-NH4+. This product meets the stringent purity requirements for use in HPLC analysis, ensuring consistent and reliable chromatographic separations.

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2 protocols using hplc grade ammonium formate

1

Simultaneous Quantification of Esomeprazole, Rabeprazole, and Levosulpiride

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Reference standards of esomeprazole magnesium hydrate, rabeprazole sodium, levosulpiride and lansoprazole (internal standard, IS) were purchased from Sigma-Aldrich (Hyderabad, India). HPLC grade ammonium formate was procured from Thermo Fisher Scientific (Mumbai, India). HPLC grade methanol and acetonitrile were procured from J.T Baker (Phillipsburg, USA). Milli-Q water (18.2 mΩ and TOC≤50 ppb) generated using a Milli-Q purification system (Millipore, Bangalore, India) was used throughout the study. Methyl tert-butyl ether and ethyl acetate were purchased from Rankem (Hyderabad, India). Drug-free human plasma was procured from King George Hospital (Visakhapatnam, India).
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2

Isolation and Characterization of Bioactive Saikosaponins from Iodes hainanensis

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Saikosaponin A (internal standard, IS) was obtained from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China, 110777-201510). IhD, IsA, ID and IA were isolated from I. hainanensis in our previous studies.16–18 Their structures were showed in Fig. 1 and the purity of all chemicals were determined to be more than 98% by HPLC.
The leaves of I. hainanensis were collected from Guangxi province of China in October 2015 and were identified and authenticated by Ke Zan (National Institutes for Food and Drug Control, Beijing, China). A voucher specimen (no. 20151101) was deposited at School of Traditional Chinese Medicine, Capital Medical University, China.
HPLC-grade ammonium formate, formic acid and ethyl acetate were purchased from Thermo Fisher Scientific (Hudson, NH, USA). Methanol (LC-MS grade) was purchased from Thermo Fisher Scientific (Hudson, NH, USA). Deionized water prepared by the Millipore system (Molsheim, France) was used for all the preparations. Column chromatography was performed on Macroporous resin D101 (Tianjin Haiguang Chemical Co., Ltd., Tianjin, P. R. China), polyamide (30–60, Shanghai Aladdin Industrial Co., Ltd., Shanghai, P. R. China) and Sephadex LH-20 (Pharmacia Fine Chemical Co., Ltd., Germany). Other reagents were of analytical grade.
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