Phase contrast microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Phase Contrast Microscope is an optical microscope that uses phase contrast to enhance the contrast of transparent and colorless specimens, such as living cells in culture. It allows for the visualization of these specimens without the need for staining or other sample preparation. The core function of the Phase Contrast Microscope is to provide high-contrast images of transparent and low-contrast samples.

Automatically generated - may contain errors

Lab products found in correlation

Inverted infinity, by Thermo Fisher Scientific (2 mentions) Hemocytometer, by Hausser Scientific (1 mentions) Hema 3, by Thermo Fisher Scientific (1 mentions) Trypan blue dye, by Merck Group (1 mentions) Hemacytometer, by Hausser Scientific (1 mentions) Megacult c medium, by STEMCELL (1 mentions) Evos xl core cell imaging system, by Thermo Fisher Scientific (1 mentions) Mycoalert plus mycoplasma detection kit, by Lonza (1 mentions)

5 protocols using phase contrast microscope

Evaluating Hematopoietic Progenitor Colonies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human HSCs (Lin⁻CD34⁺CD38⁻CD49f⁺) and MPPs (Lin⁻CD34⁺CD38⁻CD49f⁻) were FACS-sorted and cultured in rhTPO-free Megacult-C medium (Stem Cell Technologies) to assess colony formation of megakaryocyte progenitors, according to manufacturer’s instructions. Colonies were scored after 10–12 days using Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). To assess the colony formation of myeloid and erythroid progenitors, stem and progenitor cells were plated in HSC003 (human) or HSC007 (mouse) methylcellulose medium according to the manufacturer’s recommendation (R&D Systems). Colonies were scored after 14–16 days for human and 8–12 days for mouse using Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). In the serial replating assay, methylcellulose medium from primary plating was dissolved in PBS to dissociate the colonies into a single cell suspension. Subsequently, 20,000 cells from the previous plating were serially replated in 1 mL of HSC003 or HSC007 medium for human and mouse cells, respectively.

Kao Y.R., Chen J., Narayanagari S.R., Todorova T.I., Aivalioti M.M., Ferreira M., Ramos-Marques P., Pallaud C., Mantzaris I., Shastri A., Bussel J.B., Verma A., Steidl U, & Will B. (2018). Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopag. Science translational medicine, 10(458), eaas9563.

+ Open protocol

+ Expand

Bronchoalveolar Lavage Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bronchoalveolar lavage fluid (BALF) was collected by lavaging lungs with 1.0 mL of sterile PBS. Cells were isolated by centrifugation, and total cell counts were determined using a hemocytometer (Hausser Scientific) on an Inverted Infinity and Phase Contrast Microscope (Fisher Scientific). Differential cell counts were obtained via cytospins using Hema3 stained (Fisher Scientific) total cells. Differentials were performed on a minimum of 300 cells/animal.

Chamberlain N., Korwin-Mihavics B.R., Nakada E.M., Bruno S.R., Heppner D.E., Chapman D.G., Hoffman S.M., van der Vliet A., Suratt B.T., Dienz O., Alcorn J.F, & Anathy V. (2019). Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox Biology, 22, 101129.

+ Open protocol

+ Expand

Cell Viability Assessment by Trypan Blue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Briefly, cells were harvested using trypsin and stained with 0.4% trypan blue dye (Sigma-Aldrich). Trypan blue-positive and -negative cells were counted using a hemacytometer (Hausser Scientific, Horsham, PA) under a phase-contrast microscope (Fisher Scientific, Pittsburgh, PA). The results of each assay were expressed in terms of the percentage of dead cells relative to the total number of cells. Individual experiments were performed in triplicate.

Chang Y.W., Su C.M., Su Y.H., Ho Y.S., Lai H.H., Chen H.A., Kuo M.L., Hung W.C., Chen Y.W., Wu C.H., Chen P.S, & Su J.L. (2014). Novel peptides suppress VEGFR-3 activity and antagonize VEGFR-3-mediated oncogenic effects. Oncotarget, 5(11), 3823-3835.

+ Open protocol

+ Expand

Resveratrol-Induced Morphological Changes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were exposed to 50 μM or 100 μM of RES for 16 hours. Media were removed, and after media removal, cells were washed with DPBS and then suspended in 50 μl of DPBS. To assess morphological changes, cells were observed under a phase-contrast microscope (Life Tech, Grand Island, NY).

Kumar S., Eroglu E., Stokes JA I.I.I., Scissum-Gunn K., Saldanha S.N., Singh U.P., Manne U., Ponnazhagan S, & Mishra M.K. (2017). Resveratrol induces mitochondria-mediated, caspase-independent apoptosis in murine prostate cancer cells. Oncotarget, 8(13), 20895-20908.

+ Open protocol

+ Expand

Cell Culture of DMS114 and RT112 Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

DMS114 cell line was cultured in Waymouth’s medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin (100 U/mL) and streptomycin (100 µg/mL). Similarly, RT112 cells were grown in RPMI-1640 and 2mmol/L L-glutamine. Cells were grown as monolayer cultures and maintained in a humidified atmosphere of 5% CO₂ in air at 37°C. Cell morphology was monitored using an EVOS XL Core Cell Imaging System under a phase-contrast microscope (Life Technologies, Pittsburgh, PA, USA). Cells were routinely tested for mycoplasma contamination monthly using the MycoAlert Plus Mycoplasma detection kit (Lonza, Walkersville, MD, USA) following manufacturer’s protocol. Cells were tested last during May 2016.

Datta J., Damodaran S., Parks H., Ocrainiciuc C., Miya J., Yu L., Gardner E.P., Samorodnitsky E., Wing M.R., Bhatt D., Hays J., Reeser J.W, & Roychowdhury S. (2017). Akt Activation Mediates Acquired Resistance to Fibroblast Growth Factor Receptor Inhibitor BGJ398. Molecular cancer therapeutics, 16(4), 614-624.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!