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Cd127 pe cy5 r34.34 and nkg2a pe cy7 z199

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CD127-PE/Cy5 (R34.34) is a fluorochrome-conjugated antibody used for the detection and analysis of CD127 (Interleukin-7 receptor alpha chain) in flow cytometry applications. NKG2A-PE/Cy7 (Z199) is a fluorochrome-conjugated antibody used for the detection and analysis of NKG2A (Natural Killer Group 2, member A) in flow cytometry applications.

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Lab products found in correlation

Anti cd117 bv421 and pe cy7 104d2, by BioLegend (2 mentions) Anti il1r1 pe fab269p, by R&D Systems (2 mentions) Anti eomes pe eflour 610 wd1928, by Thermo Fisher Scientific (2 mentions) Anti rorγt pe clone afkjs 9, by Thermo Fisher Scientific (2 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CD127-PE (12 citations) NKG2A-PE (10 citations) NKG2A (Z199, (6 citations) NKG2a-PE-Cy7 (5 citations)

2 protocols using cd127 pe cy5 r34.34 and nkg2a pe cy7 z199

1

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
Bennstein S.B., Weinhold S., Degistirici Ö., Oostendorp R.A., Raba K., Kögler G., Meisel R., Walter L, & Uhrberg M. (2021). Efficient In Vitro Generation of IL-22-Secreting ILC3 From CD34+ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche. Frontiers in Immunology, 12, 797432.
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2

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
Bennstein S.B., Weinhold S., Degistirici Ö., Oostendorp R.A., Raba K., Kögler G., Meisel R., Walter L, & Uhrberg M. (2021). Efficient In Vitro Generation of IL-22-Secreting ILC3 From CD34+ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche. Frontiers in Immunology, 12, 797432.
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