Anti-OVA IgE clone 2C6 [9 (link)] was made in the UCSF Monoclonal Antibody Core. The antibody was then conjugated using a Life Technologies Alexa Fluor 647 Antibody Labeling Kit. Additional antibodies included R&D AF3767 goat anti-MMCP6 and Jackson ImmunoResearch Alexa 488 donkey anti-goat IgG 705-165-147. Dextrans, 10,000 MW (Life Technologies) labeled with Texas Red or Alexa 647, were also used.
Dextrans
Manufactured by Thermo Fisher Scientific
Dextrans are a group of polysaccharides produced by the bacterium Leuconostoc mesenteroides. They are widely used as laboratory reagents and in various pharmaceutical and biotechnological applications. Dextrans have a linear backbone of α-1,6-linked D-glucose units with varying degrees of branching through α-1,2, α-1,3, or α-1,4 linkages. The molecular weight and degree of branching can be controlled during the manufacturing process, making dextrans versatile molecules for diverse research and industrial purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dextrans
1
Conjugation and Labeling of Antibodies
2
Dextran Injection for Hemolymph Tracing in Flies
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Live animals were injected with fluorescently labeled 3,000 MW Dextrans (Life Technologies, Grand Island, NY, Texas Red, lysine conjugated (cat. # D-3328) or neutrally charged (cat. # D-3329); results did not differ). Dextrans were dissolved in 1× PBS at 10 mg/ml, centrifuged 5 min to remove undissolved dye, aliquoted, stored at −20°C. Flies were anesthetized on CO2 and mounted on agarose. A volume of 20–50 nl Dextrans were injected into the abdominal hemolymph with an Eppendorf FemtoJet and Femtotip needles (Eppendorf NA, Hauppauge, NY) (Supplementary Figure S1 shows exact position). Fluorescence in the wing vein hemolymph was observed in real time with epifluorescence microscopy on a Leica DM6000B compound microscope.
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