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Ms1096 gal4

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MS1096-GAL4 is a Drosophila melanogaster (fruit fly) genetic tool that expresses the GAL4 transcriptional activator in the wing imaginal disc. It is commonly used in Drosophila research to manipulate gene expression in the developing wing.

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Lab products found in correlation

Gmr gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas egfp, by BD (1 mentions) Uas mcd8 gfp, by BD (1 mentions) Da gal4, by BD (1 mentions) Oregon r, by Bloomington Drosophila Stock Center (1 mentions) Tub gal80ts, by BD (1 mentions) Mex gal4, by BD (1 mentions) Hs flp, by BD (1 mentions) En gal4, by BD (1 mentions)

3 protocols using ms1096 gal4

1

Drosophila Genetic Manipulations

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All Drosophila strains were maintained at 25 °C. w1118 strains were used for wild-type control. Following Drosophila strains were used in this study: GMR-GAL4 (Bloomington Drosophila Stock Center [BDSC] 9146), MS1096-GAL4 (BDSC 8860), heat shock-GAL4 (BDSC BL2077), Hedgehog-GAL4 (BDSC 67046), UAS-eGFP (BDSC 5430), UAS-Pod1 (BDSC 8801), UAS-pod1RNAi (VDRC 108886), UAS-HippoRNAi (from Dr Georg Halder), UAS-Yorkie:GFP and UAS-YorkieS168A:GFP (from Dr Ken Irvine), UAS-Sd (BDSC 9374), and UAS-Src64BRNAi (BDSC 62157).
Park J., Jun K., Choi Y., Yoon E., Kim W., Jang Y.G, & Chung J. (2020). CORO7 functions as a scaffold protein for the core kinase complex assembly of the Hippo pathway. The Journal of Biological Chemistry, 296, 100040.
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2

Drosophila Manf Overexpression Study

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Fly stocks and crosses were maintained at 25°C. The following fly lines were used in the study: w−, UAS-DmManf133 (line L3), UAS-DmManf135 (line L5) and DmManfΔ96/TM6 Tb Sb EYFP [14 (link)], 69B-GAL4 (Bloomington Drosophila Stock Center (BDSC) #1774) [31 (link)], da-GAL4 (BDSC #5460) [32 (link)], MS1096-GAL4 (BDSC #8860) [33 (link)], tub-GAL4/TM6 Tb Sb EYFP (BDSC #5138) [34 (link)], UAS-mCD8-GFP (BDSC #5130) [34 (link)], UAS-Hsc3 (BDSC #5843) [35 (link)]. T(2;3)SM6a-TM6B Tb translocation balancer (originating from pnutXP/T(2;3)SM6a-TM6B Tb, BDSC #5687) was used in viability studies (referred as SM6-TM6). UAS-RNAi lines (listed in S1 Table) were obtained from BDSC [36 (link)] and Vienna Drosophila RNAi Center [37 (link)]. Adult flies were imaged with ProgRes SpeedXT camera (Jenoptik). Genes were annotated according to Flybase [38 (link)].
Lindström R., Lindholm P., Kallijärvi J., Palgi M., Saarma M, & Heino T.I. (2016). Exploring the Conserved Role of MANF in the Unfolded Protein Response in Drosophila melanogaster. PLoS ONE, 11(3), e0151550.
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3

Drosophila Lineage-Specific Gene Expression

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We used Oregon-R (Bloomington Drosophila Stock Center (BDSC) 6362) as a wild-type strain. For cell lineage specific gene expression, we utilized the Gal4-UAS system, in which transcriptional activator Gal4 is expressed in a specific cell lineage and binds to UAS (upstream activating sequence) to induce downstream gene expression. The following Gal4 lines were used: NP1-Gal4 (Kyoto stock center 112001), mex-Gal4 (a gift from Dr. Lucy Erin O'Brien), esg-Gal4 (a gift from Dr. Shigeo Hayashi), Dl-Gal4 (a gift from Dr. Xiankun Zeng), MS1096-Gal4 (BDSC 8860), and en-Gal4 (BDSC 25752). tub-Gal80 ts (BDSC 7019) was used to temporally regulate Gal4-driven gene expression. To induce mosaic gene expression, tub-FRT-Gal80 (BDSC 38880) and hs-FLP (BDSC 1929) were used. For the split-lacZ analysis, flies with the following genotype were used: hs-FLP; mex-Gal4, X15-33 and tub-Gal80ts, X15-29 Diptericin-lacZ reporter (BDSC 55707) was used to histologically examine the AMP expression by X-gal staining. Bloomington Deficiency Kit (BDSC, http://flystocks.bio.indiana.edu/ Browse/df/dfkit-info.htm) was utilized for the genome-wide screen to identify gene deletions that alleviate the mortality of NP1-Gal4-mediated Atg7 RNAi flies upon DSS feeding.
Nagai H., Tatara H., Tanaka-Furuhashi K., Kurata S, & Yano T. (2021). Homeostatic Regulation of ROS-Triggered Hippo-Yki Pathway via Autophagic Clearance of Ref(2)P/p62 in the Drosophila Intestine. Developmental cell, 56(1).
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