Descriptive and inferential analyses were performed with SPSS Statistic Software version 22.0 (Armonk, NY, IBM Corp.). Statistical figures were prepared using RStudio Version April 1, 1717 (RStudio Team (2021). RStudio: Integrated Development Environment for R. RStudio, PBC, Boston, MA, USA; URL http://www.rstudio.com/ ).
Probit regression analysis was used to calculate LOD for analytical sensitivity. Pearson's Chi-square (χ2) was used to assess statistical differences between qualitative variables (genotype distribution, and genotype with sex, risk factors, location of lesions, simple or multiple lesions and prior treatment), and the t-test was used to calculate mean differences (age and time since onset) with other factor variables. The Kappa (κ) statistic was applied to calculate the level of agreement for the precision (inter and intra-run) assays. All CI were 95 %.
Probit regression analysis was used to calculate LOD for analytical sensitivity. Pearson's Chi-square (χ2) was used to assess statistical differences between qualitative variables (genotype distribution, and genotype with sex, risk factors, location of lesions, simple or multiple lesions and prior treatment), and the t-test was used to calculate mean differences (age and time since onset) with other factor variables. The Kappa (κ) statistic was applied to calculate the level of agreement for the precision (inter and intra-run) assays. All CI were 95 %.