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Optima grade formic acid

Manufactured by Thermo Fisher Scientific
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Optima-grade formic acid is a high-purity chemical reagent manufactured by Thermo Fisher Scientific. It is a clear, colorless liquid with the chemical formula HCOOH. Optima-grade formic acid is designed for use in various analytical and research applications that require a reliable and consistent source of this chemical compound.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (8 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Laboratory water purification system, by Merck Group (2 mentions) Thc toxbox kit, by Cayman Chemical (2 mentions) Isolute sle 48 well plates, by Biotage (2 mentions) Borane pyridine, by Merck Group (2 mentions) Glacial acetic acid, by Merck Group (2 mentions) Formaldehyde, by Merck Group (2 mentions) Acetone, by Thermo Fisher Scientific (1 mentions) Barnstead nanopure diamond water purification system, by Thermo Fisher Scientific (1 mentions) Leucine enkephalin, by Waters Corporation (1 mentions) Sodium phosphate, by Thermo Fisher Scientific (1 mentions) Calcium chloride dihydrate, by Thermo Fisher Scientific (1 mentions) Guanidine hydrochloride, by Merck Group (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Anhydrous sodium acetate, by Merck Group (1 mentions) Sequencing grade trypsin, by Promega (1 mentions) Formaldehyde d2, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions)

9 protocols using optima grade formic acid

1

Quantitative Analysis of Coffee Phenolics

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Optima-grade formic acid, acetonitrile, methanol, acetone, and food-grade hydrochloric acid (HCl) were purchased from Fisher Scientific (Waltham, MA, USA). Quinic acid, methylparaben, deuterated methanol, and deuterated water were purchased from Millipore Sigma (Burlington, MA, USA). 3-Caffeoylquinic acid (3-CQA), 4-caffeoylquinic acid (4-CQA), and 5-caffeoylquinic acid (5-CQA) were purchased from BOC Sciences (Shirley, NY, USA). Nanopure water was purified through Barnstead Nanopure Diamond Water Purification System (Thermo Fisher, Dubuque, IA, USA). Leucine enkephalin was purchased from Waters Co. (Milford, MA, USA). Organic green Arabica and Robusta coffee beans were purchased from local suppliers (Columbus, OH, USA).
Lin H., Tello E., Simons C.T, & Peterson D.G. (2022). Identification of Non-Volatile Compounds Generated during Storage That Impact Flavor Stability of Ready-to-Drink Coffee. Molecules, 27(7), 2120.
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2

Purification and Analysis of Human IgG3

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Tris(hydroxylmethyl)aminomethane (Trizma) base, guanidine hydrochloride, sodium acetate anhydrous, acetonitrile, N-Ethylmaleimide, and Gamma-globulins from human serum were purchased from Sigma-Aldrich (St. Louis, MO). High capacity Protein A resin, sodium phosphate, glycine hydrochloride, sodium chloride, calcium chloride dihydrate, and optima grade formic acid were from Fisher Scientific (Pittsburgh, PA). Native human IgG3 (with λ light chain) was purchased from Fitzgerald (Acton, MA), mouse anti-human IgG3 antibody was purchased from Invitrogen (ThermoFisher Scientific, Grand Island, NY), sequencing grade trypsin was acquired from Promega (Madison, WI), and Protein G resin was prepared in-house.
Lakbub J.C., Clark D.F., Shah I.S., Zhu Z., Go E.P., Tolbert T.J, & Desaire H. (2016). Disulfide Bond Characterization of Endogenous IgG3 Monoclonal Antibodies Using LC-MS: An Investigation of IgG3 Disulfide-mediated Isoforms. Analytical methods : advancing methods and applications, 8(31), 6046-6055.
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3

High-Throughput THC Quantification Kit

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The commercially available THC ToxBox® kit provided by Cayman Chemical Company (Ann Arbor, MI) or PinPoint Testing, LLC (Little Rock, AR) streamlines sample preparation and testing procedures to allow high-throughput testing capacity (Figures 2 and 3). This kit incorporates NIST-traceable, certified reference material for all standards and isotopically-labeled internal standards (THC-d3, THC-OH-d3, and THC-COOH-d9) to control for extraction efficiencies. The kit also includes ISOLUTE® SLE+ 48-well plates manufactured by Biotage (Charlotte, NC). Optima-grade formic acid, acetonitrile, and methanol were purchased from Fisher Scientific (Fairlawn, NJ). Deionized water was purified to 18.2 MΩ•cm resistivity using the equivalent of a Millipore laboratory water purification system. All other chemicals and supplies were provided by Cerilliant (Round Rock, TX), Cayman Chemical Company, Biotage or HemoStat Laboratories (Dixon, CA). Blank defibrinated sheep blood or blank human blood void of THC, THC-OH, and THC-COOH contamination was used for all studies.
Patton A.L., Jones J.O., Nord A., Eversole D., Feazell E.E., Mauldin K., Li L., Williams L.D., Bai S., Channell K., Endres G., Gamette M, & Moran J.H. (2018). Multi-laboratory validation of a Δ9-tetrahydrocannabinol LC-MS/MS test kit designed for quantifying THC and marijuana metabolites in blood. Forensic science and criminology, 3(1), 10.15761/FSC.1000125.
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4

Proteomic Analysis Sample Preparation

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Methanol, glacial acetic acid, borane pyridine, formaldehyde, formaldehyde-d2, Dithiothreitol (DTT) and iodoacetamide (IAA) were obtained from Sigma-Aldrich (St. Louis, MO). Optima grade formic acid, acetonitrile (ACN), water, and Methanol were purchased from Fisher Scientific (Pittsburgh, PA).
Jia C., Yu Q., Wang J, & Li L. (2014). Qualitative and Quantitative Top-down Mass Spectral Analysis of Crustacean Hyperglycemic Hormones in Response to Feeding. Proteomics, 14(10), 1185-1194.
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5

Mass Spectrometry Reagent Preparation

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Glacial acetic acid, borane pyridine, formaldehyde, 2,5-dihydroxybenzoic acid (DHB), and ammonium bicarbonate were obtained from Sigma-Aldrich (St. Louis, MO). Optima grade formic acid, acetonitrile (ACN), water, and methanol (MeOH) were purchased from Fisher Scientific (Pittsburgh, PA).
Ye H., Wang J., Zhang Z., Jia C., Schmerberg C., Catherman A.D., Thomas P.M., Kelleher N.L, & Li L. (2015). Defining the Neuropeptidome of the Spiny Lobster Panulirus interruptus Brain Using a Multidimensional Mass Spectrometry-Based Platform. Journal of proteome research, 14(11), 4776-4791.
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6

Capillary-based Analytical Techniques

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Optima grade water, Optima grade 0.1% formic acid in water, Optima grade 0.1% formic acid in acetonitrile,
Optima grade formic acid and HPLC grade acetonitrile were obtained from Fisher Scientific (Fairlawn, NJ). HPLC grade acetone was
obtained from Sigma-Aldrich (St. Louis, MO). Formamide (98%) was obtained from Aldrich (Milwaukee, WI) and Kasil 1 (Kasil)
was obtained from PQ Corporation (Valley Forge, PA). An Impulse Stripall® Thermal Wire Stripper (model
#TWC-1) with a Thermal Controller and mounting was obtained from Telodyne (San Diego, CA). Polyimide coated fused-silica
(FS) tubing of various dimensions (15 μm – 180 μm id x 360 μm od) was obtained from Polymicro
Technologies (Phoenix, AZ). Unless otherwise noted the various valves, fittings and stainless steel (SS) tubing were obtained from
Valco Instruments (Houston, TX).
Mozziconacci O., Stobaugh J.T., Bommana R., Woods J., Franklin E., Jorgenson J.W., Forrest M.L., Schöneich C, & Stobaugh J.F. (2017). Profiling the Photochemical-Induced Degradation of Rat Growth Hormone with Extreme Ultra-pressure Chromatography-Mass Spectrometry Utilizing Meter-Long Microcapillary Columns Packed with Sub-2-μm Particles. Chromatographia, 80(9), 1299-1318.
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7

High-Throughput THC Quantification Kit

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The commercially available THC ToxBox® kit provided by Cayman Chemical Company (Ann Arbor, MI) or PinPoint Testing, LLC (Little Rock, AR) streamlines sample preparation and testing procedures to allow high-throughput testing capacity (Figures 2 and 3). This kit incorporates NIST-traceable, certified reference material for all standards and isotopically-labeled internal standards (THC-d3, THC-OH-d3, and THC-COOH-d9) to control for extraction efficiencies. The kit also includes ISOLUTE® SLE+ 48-well plates manufactured by Biotage (Charlotte, NC). Optima-grade formic acid, acetonitrile, and methanol were purchased from Fisher Scientific (Fairlawn, NJ). Deionized water was purified to 18.2 MΩ•cm resistivity using the equivalent of a Millipore laboratory water purification system. All other chemicals and supplies were provided by Cerilliant (Round Rock, TX), Cayman Chemical Company, Biotage or HemoStat Laboratories (Dixon, CA). Blank defibrinated sheep blood or blank human blood void of THC, THC-OH, and THC-COOH contamination was used for all studies.
Patton A.L., Jones J.O., Nord A., Eversole D., Feazell E.E., Mauldin K., Li L., Williams L.D., Bai S., Channell K., Endres G., Gamette M, & Moran J.H. (2018). Multi-laboratory validation of a Δ9-tetrahydrocannabinol LC-MS/MS test kit designed for quantifying THC and marijuana metabolites in blood. Forensic science and criminology, 3(1), 10.15761/FSC.1000125.
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8

Quantitative Kynurenine Pathway Analysis

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KYNA, L-kynurenine (“kynurenine”), 3-hydroxykynurenine (3-HK), aminooxyacetic acid (AOAA), NAC, GSH, MS grade porcine trypsin and Millipore C18 ZipTips were purchased from Sigma (St. Louis, MO, USA). Endoproteinase Glu-C, LC-MS grade water, acetonitrile, and Optima grade formic acid were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Other chemicals were obtained from a variety of suppliers, as specified below, and were of the highest commercially available purity. All chemicals used in enzyme assays, including kynurenine, NAC and GSH, were dissolved in saline, and the solutions were adjusted to pH 6.8 before experimental use.
Ayala T.B., Sathyasaikumar K.V., Uys J.D., Peréz-de-la-Cruz V., Pidugu L.S, & Schwarcz R. (2020). N-Acetylcysteine Inhibits Kynurenine Aminotransferase II. Neuroscience, 444, 160-169.
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9

Quantitative Metabolic Profiling Analysis

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All chemicals and solvents used in this study were high purity (>97% or better). Chemical standards for HVA, Glu, Lac, Pyr, MetSO, NAA, AA, LA, isotopically-labeled internal standards glutamic acid-13C5,15N (Glu-13C5,15N), 5-hydroxyindoleacetic acid 4,6,7 D3-acetic-3-D2 (5-HIAA-D5), and N-acetyl-L-aspartic acid-2,3,3-D3 (NAA-D3) were purchased from Sigma-Aldrich, (St. Louis, MO, USA). In addition, 5-HIAA, F2α, LC-MS-grade methanol, isopropanol, acetonitrile (ACN), and optima™ grade formic acid (FA) were purchased from Fischer Scientific (Hampton, NH, USA). Isotopically labeled internal standard F2α-isoprostane-D4 (F2α-D4) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Ultrapure water (18.2 MΩ·cm) was generated in-house by a Millipore Elix-3 purification system (Millipore, Billerica, MA, USA).
Sigler A., Wu J., Pfaff A., Adetunji O., Nam P., James D., Burton C, & Shi H. (2023). Repeated Low-Level Blast Exposure Alters Urinary and Serum Metabolites. Metabolites, 13(5), 638.
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