The ability of inhibitors to induce apoptosis in neuroblastoma cell lines was determined using an Annexin-V:APC flow cytometry assay kit (BD Biosciences, CA) following the manufacturer's instructions. Briefly, 0.3 × 106 cells/ml neuroblastoma cell lines were plated in 12-well plates and cultured and treated with inhibitors alone or in combination with topotecan for 72 hours. The percent of the cells undergoing apoptosis was then assessed using Annexin-V/propidium iodide double staining.
Annexin 5 apc flow cytometry assay kit
Manufactured by BD
The Annexin-V:APC flow cytometry assay kit is a laboratory reagent used to detect and quantify apoptosis, or programmed cell death, in cell samples. The kit contains Annexin-V conjugated to the fluorescent dye allophycocyanin (APC), which binds to phosphatidylserine exposed on the surface of apoptotic cells. This allows for the identification and enumeration of apoptotic cells through flow cytometry analysis.
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2 protocols using annexin 5 apc flow cytometry assay kit
1
Apoptosis in Neuroblastoma Cells
2
Apoptosis Induction in Medulloblastoma Cell Lines
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The ability of inhibitors to induce apoptosis in MB cell lines was determined using an Annexin-V:APC flow cytometry assay kit (BD Biosciences, CA) following the manufacturer's instructions. Briefly, 0.3 × 106 cells/ml MB cell lines were plated in 12-well plates and treated with inhibitors alone or in combination for 72 hours. The percent of the cells undergoing apoptosis was then assessed using Annexin-V/propidium iodide double staining. In some experiments, the induction of apoptosis by inhibitors was also determined using Caspase 3/7 activity assay kit (Promega, WI) following the manufacturer's instructions.
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