All reagents used in this experiment were at least analytical grade. All the ultrapure water used in the experiment was obtained through Milli-Q (Millipore, Bradford, MA, USA). Ten sulfonamides: sulfamethoxazole (SMZ), sulfafurazole (SIZ), sulfamethizole (SMT), sulfadiazine (SD), sulfadimidine (SM2), sulfametoxydiazine (SMD), sulfadoxine (SDM′), sulfapyridine (SPD), sulfathiazole (ST), sulfamethoxypyridazine (SMP) were purchased from Sigma. 1,3,5-Triformylphloroglucinol (Tp), glycol, diglycolic anhydride (DA), 1,6-hexanediamine, benzidine (BD), and FeCl3·6H2O were purchased from Aladdin (Shanghai, China). Ethanol, N,N-dimethylformamide (DMF), tetrahydrofuran (THF), mesitylene, and 1,4-dioxane were purchased from Concord Chemical Research Institute (Tianjin, China). HNO3 were obtained from Guangfu Fine Chemical Research Institute (Tianjin, China). All glassware should be soaked in aqua regia (HCl : HNO3 = 3 : 1, v/v) for at least 24 h and cleaned with ultrapure water.
Sulfadimidine
Manufactured by Merck Group
Sourced in United States
Sulfadimidine is a chemical compound used in pharmaceutical and research laboratory settings. It functions as a sulfonamide, a class of antimicrobial agents. The core function of Sulfadimidine is to serve as a laboratory reagent for various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Sulfadiazine, by Merck Group (4 mentions)
Tetracycline, by Merck Group (3 mentions)
Sulfamethoxazole, by Merck Group (2 mentions)
Sulfathiazole, by Merck Group (2 mentions)
Sulfadoxine, by Merck Group (2 mentions)
Sulfametoxydiazine, by Merck Group (2 mentions)
Lincomycin, by Merck Group (2 mentions)
Chloramphenicol, by Merck Group (2 mentions)
Trimethoprim, by Merck Group (2 mentions)
Erythromycin, by Merck Group (2 mentions)
Sulfamethizole, by Merck Group (1 mentions)
Sulfamethoxypyridazine, by Merck Group (1 mentions)
Sulfapyridine, by Merck Group (1 mentions)
Sulfafurazole, by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Spiramycin, by Merck Group (1 mentions)
Enrofloxacin, by Merck Group (1 mentions)
Doxycycline, by Merck Group (1 mentions)
Neomycin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
5 protocols using sulfadimidine
1
Determination of Sulfonamides via Coordination Polymer
2
Antibiotic Preparation and Storage Protocol
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β–lactam: penicillin G (PEN), cefquinome (CEF); aminoglycosides: neomycin (NEO), streptomycin (STR); tetracyclines: doxycycline (DOX), tetracycline (TET); macrolides: erythromycin (ERY), spiramycin (SPI); sulfonamides: sulfadiazine (SDZ), sulfadimidine (SDM); lincosamides: lincomycin (LIN); quinolones: danofloxain (DAN), enrofloxacin (ENR); trimethoprim (TMP); and chloramphenicol (CAP) were all purchased from Sigma-Aldrich (St. Louis, MO, United States). Drugs for the preparation of antimicrobial solutions were stored and handled according to the manufacturers’ instructions before use. In addition, the methods for the preparation of stock solutions and working standard solutions of antibiotics were shown in Table 1 .
3
Simultaneous Determination of Antibiotics
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The chemical reagents employed were commercially available. Methanol (MeOH), acetonitrile (ACN), formic acid (FA) and water as mobile phase of LC-MS pure grade were purchased from Merck (Shanghai, China). Analytical grade solvents were purchased from Guangzhou Chemical Regent Factory (Guangzhou, China). Other reagents were all purchased from Aladin (Shanghai, China). The sieve plates for SPE column were made in polypropylene with the pore size of 20 μm.
The standard of nine single antibiotic, sulfadiazine (SDZ), sulfadimidine (SMZ), tetracycline (TC), sulfametoxydiazine (SME), oxytetracycline (OTC), sulfadoxine (SDO), chlortetracycline (CTC), sulfachloropyridazine (SCP), doxycycline (DC) were purchased from Sigma-Aldrich (Saint Louis, USA). The stock solution of the mixed standard were prepared as 10 mg L−1 in methanol/water (1 : 1, v/v), and stored at 4 °C in darkness. Different calibration solutions were prepared daily, by dilution of the stock solution with methanol/water (1 : 4, v/v).
The standard of nine single antibiotic, sulfadiazine (SDZ), sulfadimidine (SMZ), tetracycline (TC), sulfametoxydiazine (SME), oxytetracycline (OTC), sulfadoxine (SDO), chlortetracycline (CTC), sulfachloropyridazine (SCP), doxycycline (DC) were purchased from Sigma-Aldrich (Saint Louis, USA). The stock solution of the mixed standard were prepared as 10 mg L−1 in methanol/water (1 : 1, v/v), and stored at 4 °C in darkness. Different calibration solutions were prepared daily, by dilution of the stock solution with methanol/water (1 : 4, v/v).
4
Analytical Method for Sulfonamide Antibiotics
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Ammonium molybdate tetrahydrate (H24Mo7N6O24·4H2O) (99%), thioacetamide (TAA) (98%), sulfathiazole (STZ), sulfadimidine (SDD), sulfadiazine (SDZ), sulfamethoxazole (SMZ), sulfacetamide (ST), sulfachloropyridazine (SCD), N4-phthalylsulfathiazole (PST), succinylsulfathiazole (SST), sodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), methanol for HPLC (99.9%) and acetonitrile for HPLC (99.9%) were purchased from Sigma-Aldrich (St Louis, USA). Acetone, sodium hydroxide (NaOH) and hydrochloric acid (HCl) were purchased from Beijing Chemical Works (Beijing, China). Polyethylene glycol (PEG4000) was purchased from Merck (Germany). Ultrapure water (18.2 MΩ cm) was prepared using a Milli-Q Gradient ultrapure water system (Millipore, Milford, MA, USA).
The eight SAs were dissolved in a 0.1 mol l−1 NaOH solution to prepare standard stock solutions of 2.0 mg ml−1 for each SA, and these standard solutions were stored at 4°C and protected from light. The working solutions to be analysed were diluted with ultrapure water. The calibration standards of SAs were prepared with five levels of concentration in the range of 0.3 to 30 µg ml−1.
The eight SAs were dissolved in a 0.1 mol l−1 NaOH solution to prepare standard stock solutions of 2.0 mg ml−1 for each SA, and these standard solutions were stored at 4°C and protected from light. The working solutions to be analysed were diluted with ultrapure water. The calibration standards of SAs were prepared with five levels of concentration in the range of 0.3 to 30 µg ml−1.
5
Multiplex Analysis of Veterinary Drugs
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Standard chemicals, namely, danofloxacin, erythromycin, sulfadimidine, benzylpenicillin potassium, chloramphenicol, lincomycin, tetracycline, spectinomycin, thiamphenicol, dexamethasone, kanamycin, melamine, trimethoprim, zearanol, aflatoxin M1, flunixin meglumine, and florfenicol, were purchased from Sigma (St. Louis, MO, USA). All the chemicals used were of analytical grade. Lead acetate, potassium oxalate, disodium hydrogen phosphate, trichloroacetic acid, sodium hydroxide, and dichloromethane were obtained from Sinopharm Chemical Reagent Co., Ltd. (China). High-purity water was obtained from a Milli-Q water system (Millipore, Billerica, MA, USA). Pure milk was purchased from a local supermarket. Commercialized semiquantitative ICA strip and an ICA strip reader were supplied by Beijing Meizheng Bio-Tech Co., Ltd. (Beijing, China)
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