Diluted PRP (3 × 108 plt/ml in platelet-poor plasma) was placed in an aggregometer cuvette at 37°C and stirred. Agonists were added (ADP, arachidonic acid, and epinephrine were purchased from Helena Laboratories; collagen was purchased from Bio/Data Corporation; TRAP-14 was obtained from Polypeptide group; and ristocetin was obtained from Stago) at concentrations given in the text. Light transmission was recorded on an APACT 4004 optical aggregation system (Labor BioMedical Technologies GmbH).
Ristocetin
Manufactured by Stago
Sourced in France
Ristocetin is a laboratory reagent used to assess the functioning of von Willebrand factor (vWF) in blood samples. It is used in various coagulation tests to evaluate the ability of vWF to bind to platelet receptors, which is a crucial step in the blood clotting process.
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Lab products found in correlation
2 protocols using ristocetin
1
Platelet aggregation assay
2
Platelet Aggregation Testing in Citrated PRP
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Platelet aggregationwas tested in citrated platelet-rich plasma (PRP) using 1.2 mg/mL ristocetin (Stago, Asnières-sur-Seine, France), 5-μM adenosine diphosphate (ADP) (Sigma Aldrich Chimie, Lyon, France), 1-mM arachidonic acid (AA; Nu Chek Prep, Elysian, MN), 25-μM thrombin receptor activating peptide (TRAP; NeosystemSA, Strasbourg, France), 4-μMepinephrine (Helena Laboratories, Beaumont, TX), and 2 μg/μL Horm equine tendon collagen (Nycomed Pharma, Unterschleibheim, Germany) in an APACT 4004 aggregometer (Elitech, Salon de Provence, France) according to standard procedures15 and during 300 seconds of aggregation test time. In the case of normal platelet counts, test results were compared with reference values of 30 healthy controls. Alternatively, when patients had thrombocytopenia, platelet aggregation testswere visually analyzed by hematology experts to confirm the presence of an abnormal profile.
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