Western lighting plus ecl chemiluminescent reagents

Manufactured by PerkinElmer

Sourced in United States

The Western Lighting Plus-ECL Chemiluminescent Reagents are a set of reagents designed for the detection and quantification of proteins in Western blot analysis. They provide a chemiluminescent signal that can be used to visualize and analyze protein expression levels.

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ECL reagent (117 citations) ECL Plus (66 citations) Western Lighting Plus-ECL (55 citations) ECL Plus reagent (26 citations) Western Lighting Plus ECL reagents (12 citations) Chemiluminescent reagent (12 citations) Western Lighting Plus (7 citations)

2 protocols using western lighting plus ecl chemiluminescent reagents

RT-qPCR and Western Blot Analysis

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Total RNA was isolated using TRIzolTM (Invitrogen), and cDNA was synthesized using 2 μg of total RNA with SuperScript^® III First-Strand Synthesis System, according to the manufacturer’s protocol (Invitrogen). Real-time PCR was performed on LightCycler 480^® (Roche), using SYBR Green I Master Mix. The primer set sequences are provided in Supplementary Table S1.
Total cell lysates were prepared, and western blot analyses were conducted as described previously [32 (link)]. Antibodies are listed in Supplementary Table S2. Signals were visualized with Western Lighting Plus-ECL Chemiluminescent Reagents (Perkin Elmer, Waltham, MA, USA), and the intensity of the resulting bands was quantified by ImageJ software (NIH, Bethesda, MD, USA).

Cho H.J., Lee Y.S., Kim D.A., Moon S.A., Lee S.E., Lee S.H, & Koh J.M. (2022). Lumican, an Exerkine, Protects against Skeletal Muscle Loss. International Journal of Molecular Sciences, 23(17), 10031.

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Probing the mTOR Signaling Pathway

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Whole-cell extracts were prepared using 2X SDS buffer supplemented with fresh protease and phosphatase inhibitors. Equal volumes of protein were fractionated by SDS-PAGE and transferred to nitrocellulose membranes. The following antibodies were used: Akt, mTOR, p-mTOR^S2448, p-mTOR^S2481, ribosomal protein S6, phospho-ribosomal protein S6^S235/236, Rictor, Raptor, TSC2, p-TSC2^S939, NFκB p50, NFκB p65, IκBα, p-IkBα^S32, and IKKα (purchased from Cell Signaling Technology, Danvers, MA). β-actin antibody was purchased from Sigma-Aldrich. Anti-rabbit secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Blots were developed using Western Lighting Plus ECL chemiluminescent reagents (Perkin Elmer, Waltham, MA) and Syngene G:Box Imaging System (Frederick, MD). Band quantification was performed using Syngene Genetools gel analysis software and band intensities normalized to β-actin intensity.

Hambright H.G., Batth I.S., Xie J., Ghosh R, & Kumar A.P. (2014). Palmatine inhibits growth and invasion in prostate cancer cell: potential role for rpS6/NFκB/FLIP. Molecular carcinogenesis, 54(10), 1227-1234.

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