All cell populations were sorted using a FACSAria™ III (BD Biosciences, Franklin Lakes, NJ), and only preparations with a purity of >95% were used for further experiments. Memory CD4+ and CD8+ T cells were sorted from peripheral blood after staining with the following antibodies: anti-CD3-PE (Biolegend, San Diego, CA), anti-CD4-APC (eBiosciences, San Diego, CA), anti-CD8-Pacific Blue (Biolegend) and anti-CD45RO FITC (Biolegend). T cells expressing specific Vβ families were sorted from bulk CSF Phytohaemagglutinin (PHA)-expanded cells after staining with the corresponding TCR Vβ-specific antibodies22 (link) (Beckman Coulter, Nyon, Switzerland). B cells from peripheral blood were sorted after staining with anti-CD19-Pacific Blue (BD).
Anti cd45ro fitc
Manufactured by BioLegend
Sourced in Canada, United States
Anti-CD45RO FITC is a fluorescently-labeled antibody that binds to the CD45RO antigen expressed on the surface of T cells. It is used for the identification and enumeration of T cells in flow cytometry applications.
Automatically generated - may contain errors
2 protocols using anti cd45ro fitc
1
Multiparametric Cell Sorting Protocol
2
Sorting and Expanding T Cell Subsets
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All cell populations were sorted using a FACSAria™ III (BD Biosciences, Franklin Lakes, NJ USA), and only preparations with a purity of >95% were used for further experiments. Memory CD4+ and CD8+ T cells were sorted from peripheral blood after staining with the following antibodies: anti-CD3-PE (Biolegend, San Diego, CA, USA), anti-CD4-APC (eBiosciences, San Diego, CA, USA), anti-CD8-Pacific Blue (Biolegend), and anti-CD45RO FITC (Biolegend) as previously described (22 (link)). T cells expressing specific TRBV families were sorted from bulk phytohemagglutinin-expanded CSF T cells after staining with the corresponding TRBV-specific antibodies (Beckman Coulter, Nyon, Switzerland), expanded and cloned as previously described (22 (link)).
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