Primary antibodies against Survivin (#2808), Apollon (#8745), Livin (#5471), Smac (#2954), cleaved caspase-8 (#9496), cleaved caspase-9 (#9505), cytochrome c (#4272), α-tubulin (#2144), COX IV (#4844), ubiquitin (P4D1, #3936) were purchased from Cell Signaling Technology (Beverly, MA). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#sc-47724) were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against NAIP (#ab25968), XIAP (#ab28151), c-IAP1 (#ab108361), c-IAP2 (#ab137393) were from Abcam (Cambridge, MA). Secondary antibodies for immunofluorescence were TRITC-conjugated donkey anti-mouse IgG and Alexafluor 488–conjugated goat anti-rabbit IgG, from Jackson ImmunoResearch Laboratories (West Grove, PA).
Antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are a laboratory tool used to detect and quantify the presence of the GAPDH protein in biological samples. GAPDH is a metabolic enzyme involved in the glycolysis pathway, and its expression is often used as a reference or housekeeping gene in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
α tubulin, by Cell Signaling Technology (1 mentions)
Survivin, by Cell Signaling Technology (1 mentions)
Cleaved caspase 9, by Cell Signaling Technology (1 mentions)
Livin, by Cell Signaling Technology (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Tritc conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Cleaved caspase 8, by Cell Signaling Technology (1 mentions)
Ubiquitin p4d1, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Recombinant human vegf a, by R&D Systems (1 mentions)
Horseradish peroxide conjugated secondary antibodies, by Santa Cruz Biotechnology (1 mentions)
Vegf c, by R&D Systems (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Matrigel, by Corning (1 mentions)
Phosphatase inhibitor cocktail, by Roche (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Mg132, by Merck Group (1 mentions)
5 protocols using antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh
1
Apoptosis Pathway Protein Analysis
2
Cytotoxicity Assay and Inflammatory Pathway Analysis
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Dulbecco’s Modified Eagle’s medium (DMEM), fetal bovine serum (FBS), glutamine, and penicillin/streptomycin were obtained from Life Technologies (Grand Island, NY, USA). 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 4′-6-diamidino-2-phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Specific antibodies against iNOS, COX-2, IKKα, phos-IKKα, I-κBα, phos-I-κBα, NF-κB, and horseradish peroxidase (HRP)-conjugated anti-goat, anti-rabbit, and anti-mouse IgG secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). All other chemicals were of the highest grade commercially available and supplied either by Merck (Darmstadt, Germany) or Sigma (St. Louis, MO, USA).
3
Endothelial Cell Signaling Pathway
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3′ sialylgalactose (3SG) and 6SG were purchased from Carbosynth Ltd. (Berkshire, UK). Recombinant human VEGF-A and VEGF-C were purchased from R&D Systems, Inc. (Minneapolis, MN). Antibodies against the phosphorylated or total forms of VEGFR-2, ERK, and Akt were purchased from Cell Signaling Technology (Danvers, MA). FITC-conjugated isolectin B4 (IB4) was purchased from Millipore-Sigma (St. Louis, MO). An antibody against PECAM1 was purchased from Agilent Dako (Santa Clara, CA). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and horseradish peroxide-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).
4
Comprehensive Antibody Characterization Protocol
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All antibodies were commercially available. The primary antibodies HIF-1α (D1S7W), HIF-2α (D6T8V), HIF-1β/ARNT (D28F3), eIF4E (C46H6), phospho-eIF4E (Ser209), nonphospho (active) β-catenin (Ser33/37/Thr41) (D13A1), β-catenin (D10A8), Cyclin D1 (92G2), c-Myc (D3N8F), and Axin2 (76G6) were obtained from Cell Signaling Technology. Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α-tubulin (B-7) were from Santa Cruz Biotechnology and K. Chen, respectively. Protease inhibitor (catalog no. 11836145001) and phosphatase inhibitor cocktail (catalog no. 4906837001) tablets were bought from Roche Diagnostics, while cell lysis buffer (catalog no. 9803) was obtained from Cell Signaling Technology. LF3 (catalog no. HY-101486) was acquired from MedChemExpress. PX478 (catalog no. T6961) and KC7F2 (catalog no. T3169) were from Topscience. Matrigel (catalog no. 356237) was obtained from Corning. MG132 (catalog no. 474790) and CoCl2 were from Sigma-Aldrich.
5
Signaling Pathway Characterization Protocol
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Tramadol hydrochloride, naloxone hydrochloride dihydrate, PGF2α and hydroxyfasudil (fasudil) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Morphine hydrochloride was obtained from Takeda Pharmaceutical Co., Ltd. (Osaka, Japan). SP600125 and SB203580 were purchased from Calbiochem-Novabiochem Co. (La Jolla, CA, USA). The antibodies against phospho-specific SAPK/JNK, SAPK/JNK, phospho-specific p44/p42 MAP kinase, p44/p42 MAP kinase, phospho-specific p38 MAP kinase, and p38 MAP kinase were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA). The antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and myosin phosphatase target subunit (MYPT) were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Chalfont, UK). Other materials and chemicals were obtained commercially. PGF2α was dissolved in ethanol. Tramadol, SP600125 and SB203580 were dissolved in dimethyl sulfoxide. Morphine was dissolved in mast cell medium (150 mM NaCl, 5 mM KCl, 5.5 mM glucose, 0.8 mM MgSO4, 1 mM CaCl2 and HEPES, pH 7.4). The maximum concentration of ethanol or dimethyl sulfoxide was 0.1%, which has no effects on the assay for OPG or the detection of the protein level using a Western blot analysis (Kuroyanagi et al., 2014 ).
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