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Rabbit anti sod2

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Rabbit anti-SOD2 is a primary antibody that recognizes the superoxide dismutase 2 (SOD2) protein. SOD2 is an enzyme that plays a key role in the body's antioxidant defense system by catalyzing the conversion of superoxide radicals to hydrogen peroxide and oxygen. This antibody can be used to detect and study the expression and localization of SOD2 in various biological samples.

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Lab products found in correlation

Sulindac, by Merck Group (2 mentions) Apocynin, by Merck Group (2 mentions) C h2dcfda, by Thermo Fisher Scientific (2 mentions) Peg catalase, by Merck Group (2 mentions) Total sod assay kit, by Beyotime (2 mentions) Rabbit anti sodl, by Cell Signaling Technology (2 mentions) Horseradish peroxidase labeled igg anti rabbit or mouse antibodies, by Cell Signaling Technology (2 mentions) O2 assay kit, by Nanjing Jiancheng (2 mentions) Peg sod, by Merck Group (2 mentions) Rabbit anti catalase, by Cell Signaling Technology (2 mentions) Rabbit anti phospho β catenin, by Cell Signaling Technology (2 mentions) Catalase assay kit, by Beyotime (2 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Rabbit anti β catenin, by Cell Signaling Technology (2 mentions) H2o2 assay kit, by Beyotime (2 mentions) Bca protein assay kit, by Beyotime (2 mentions) Rabbit anti ftl, by Abcam (1 mentions) Rabbit anti ndrg1, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti vinculin, by Cell Signaling Technology (1 mentions)

4 protocols using rabbit anti sod2

1

Western Blot Analysis of Cellular Proteins

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Standard Western blots were undertaken using RIPA (radioimmunoprecipitation assay) lysates as previously described [16 (link)]. A total of 10–20 ug of lysate was resolved via SDS-PAGE. Primary antibodies were as follows: anti-vinculin (1:5000) (Cat#4650, Cell Signalling Technology, Danvers, MA, USA), mouse anti-β-actin (1:5000) (Cat#sc-69879, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-TGM2 (1:1000) (Cat#ab2386, Abcam, Cambridge, UK), rabbit anti-FTL (Cat#ab69090, Abcam, Cambridge, UK), rabbit anti-ANXA6 (Cat#31026, Abcam, Cambridge, UK), mouse anti-MVP (Cat#ab97311, Abcam, Cambridge, UK), rabbit anti-NDRG1 (Cat#5196S, Cell Signalling Technology, Danvers, MA, USA), and rabbit anti-SOD2 (Cat#13194S, Cell Signalling Technology, Danvers, MA, USA). HRP-conjugated goat anti-mouse (Cat#1706516, Bio-Rad, Hercules, CA, USA) and goat anti-rabbit (Cat#1706515, Bio-Rad, Hercules, CA, USA) secondary antibodies were used at 1:5000 and 1:3000, respectively.
Clark K.C., Nguyen E.V., Niranjan B., Wu Y., Lim Kam Sian T.C., Horvath L.G., Taylor R.A, & Daly R.J. (2023). Cell-Type-Specific Signalling Networks Impacted by Prostate Epithelial-Stromal Intercellular Communication. Cancers, 15(3), 699.
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2

Oxidative Stress Regulation in Cells

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Reagent sources were as follows: DMEM, sulindac, ET-1, PEG-catalase, PEG-SOD, apocynin (Sigma-Aldrich, St Louis, MO); DHE, C-H2DCFDA (Invitrogen Life Technologies, Eugene, Dregon, USA); BCA protein assay kit, H2O2 assay kit, catalase assay kit and total SOD assay kits (Beyotime, Shanghai, China). O2.− assay kit (Jiancheng Bioengineering Institute, Najing, China). Antibody sources were as follows: rabbit anti-GSK-3β, rabbit anti-phospho-GSK-3β (Ser9), rabbit anti-β-catenin, rabbit anti-phospho-β-catenin (Ser33/37/Thr41), rabbit anti-SODl (#2770), rabbit anti-SOD2 (#13194), rabbit anti-catalase (#14097), horseradish peroxidase-labeled IgG anti-rabbit (or mouse) antibodies (Cell Signaling Technology, Beverly, CA, USA) and mouse anti-β-actin (Abeam, Cambridge, MA, USA).
Zhang S., Huang Q., Wang Q., Wang Q., Cao X., Zhao L., Xu N., Zhuge Z., Mao J., Fu X., Liu R., Wilcox C.S., Patzak A., Li L, & Lai E.Y. (2018). Enhanced Renal Afferent Arteriolar Reactive Oxygen Species and Contractility to Endothelin-1 Are Associated with Canonical Wnt Signaling in Diabetic Mice. Kidney & blood pressure research, 43(3), 860-871.
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3

Molecular Signaling in Hippocampal Tissue

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All rats of each group were anesthetized with ether and then were decapitated. The brain was removed rapidly and the hippocampus was dissected and put onto a box chilled with ice. After the hippocampal tissue was rinsed with PBS buffer 3 times, the hippocampal tissue was homogenized at 1: 5 (wt/vol) in an ice-cold lysis buffer with protease inhibitor cocktail and phosphatase inhibitor cocktail.
The samples were subjected to SDS-PAGE and were transferred to Polyvinylidene Fluoride (PVDF) membranes. The blots were probed with the following primary antibodies: polyclonal mouse anti β-actin (Sigma), rabbit anti-Akt (Cell Signaling), mouse anti-pAkt (Cell Signaling), rabbit anti-CREB (Cell Signaling), mouse anti-pCREB (Cell Signaling), rabbit anti-PGC1α (Cell Signaling) and rabbit anti-SOD2 (Cell Signaling), monoclonal mouse anti-Bax (Cell Signaling), monoclonal mouse anti-Bcl-2 (Cell Signaling), polyclonal rabbit anti-caspase-3 (Santa Cruz), followed by incubation with species-matched horseradish peroxidase-conjugated secondary antibodies. The blots were developed with a chemiluminescence substrate solution (Pierce) and exposed to X-ray film. The optical density of immunoreactive bands was quantified using the Quantity One software (Bio-Rad).
Jia N., Sun Q., Su Q., Dang S, & Chen G. (2016). Taurine promotes cognitive function in prenatally stressed juvenile rats via activating the Akt-CREB-PGC1α pathway. Redox Biology, 10, 179-190.
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4

Oxidative Stress Regulation in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagent sources were as follows: DMEM, sulindac, ET-1, PEG-catalase, PEG-SOD, apocynin (Sigma-Aldrich, St Louis, MO); DHE, C-H2DCFDA (Invitrogen Life Technologies, Eugene, Dregon, USA); BCA protein assay kit, H2O2 assay kit, catalase assay kit and total SOD assay kits (Beyotime, Shanghai, China). O2.− assay kit (Jiancheng Bioengineering Institute, Najing, China). Antibody sources were as follows: rabbit anti-GSK-3β, rabbit anti-phospho-GSK-3β (Ser9), rabbit anti-β-catenin, rabbit anti-phospho-β-catenin (Ser33/37/Thr41), rabbit anti-SODl (#2770), rabbit anti-SOD2 (#13194), rabbit anti-catalase (#14097), horseradish peroxidase-labeled IgG anti-rabbit (or mouse) antibodies (Cell Signaling Technology, Beverly, CA, USA) and mouse anti-β-actin (Abeam, Cambridge, MA, USA).
Zhang S., Huang Q., Wang Q., Wang Q., Cao X., Zhao L., Xu N., Zhuge Z., Mao J., Fu X., Liu R., Wilcox C.S., Patzak A., Li L, & Lai E.Y. (2018). Enhanced Renal Afferent Arteriolar Reactive Oxygen Species and Contractility to Endothelin-1 Are Associated with Canonical Wnt Signaling in Diabetic Mice. Kidney & blood pressure research, 43(3), 860-871.
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