Specimens of cells and tissue were harvested and incubated for 10 min on ice with lysis buffer. The lysates were then centrifuged at 14,000 RCF for 10 min at 4 °C, and the supernatants were collected. Equal amounts of protein samples were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer onto a polyvinylidene difluoride membrane (Millipore). Western blot analyses were performed with the relevant antibodies: claudin-18 (diluted 1:200, Thermo Fisher Scientific Inc, IL, USA), p-SPAK (diluted 1:1000, OriGene, MD, USA), phosphorylated-p38 (p-p38) (diluted 1:1000, Cell Signaling Technology, USA), total-p38 (T-p38) (diluted 1:1000, Cell Signaling Technology, USA), beta-actin (diluted 1:1000, Sigma Chemical Company, MO, USA) and GAPDH (diluted 1:1000, Thermo Fisher Scientific Inc, IL, USA).
P spak
Manufactured by OriGene
Sourced in United States
P-SPAK is a laboratory equipment product manufactured by OriGene. It serves as a tool for researchers in various scientific fields. The core function of P-SPAK is to facilitate the measurement and analysis of specific cellular signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Phosphorylated p38 p p38, by Cell Signaling Technology (2 mentions)
Total p38 t p38, by Cell Signaling Technology (2 mentions)
β actin, by Merck Group (2 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Claudin 18, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
2 protocols using p spak
1
Western Blot Analysis of Cell Signaling
2
Western Blot Protein Analysis
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Western blot analyses were performed based on a standard protocol with the relevant antibodies: claudin-18 (diluted 1:200, Thermo Fisher Scienti c Inc, IL, USA), p-SPAK (diluted 1:1000, OriGene, MD, USA), phosphorylated-p38 (p-p38) (diluted 1:1000, Cell Signaling Technology, USA), total-p38 (T-p38) (diluted 1:1000, Cell Signaling Technology, USA), beta-actin (diluted 1:1000, Sigma Chemical Company, MO, USA) and GAPDH (diluted 1:1000, Thermo Fisher Scienti c Inc, IL, USA).
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