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2 protocols using n n n n tetraethylethylenediamine

1

Synthesis of Barium Carbonate Compounds

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An aqueous solution of formaldehyde (36 wt%), barium carbonate (BaCO3) and N,N,N′,N′-tetraethylethylenediamine were purchased from Sigma-Aldrich (St. Louis, MO). Calcium hydroxide (Ca(OH)2), silver(i) oxide (Ag2O), alumina (Al2O3), imidazole, pyridine and acetylacetone were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). Barium hydroxide octahydrate (Ba(OH)2·8H2O), sodium hydroxide (NaOH), potassium hydroxide (KOH), triethylamine, 2-picoline, 3-picoline, acetic acid and ammonium acetate were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Lithium oxide monohydrate (LiOH·H2O), 2-(dimethylamino)ethanol and acetonitrile for HPLC were purchased from Kishida Chemical Co., Ltd. (Osaka, Japan). Magnesium hydroxide (Mg(OH)2), 1,4-diazabicyclo[2.2.2]octane (DABCO) and N-methylpiperidine were purchased from Kanto Chemical Co. Inc. (Tokyo, Japan). Amberlite® IR-120 H and IRA-910 Cl were purchased from Sigma-Aldrich (St. Louis, MO). Amberlite® IRA-910 Cl was used after exchanging Cl ions to OH ions (Amberlite® IRA-910 OH) by washing with 1.0 M NaOH. For thin layer chromatography (TLC), Merck precoated TLC plates (silica gel 60 F254) were used. Water was purified with a Millipore Milli-Q system. Other reagents were used without further purification.
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2

Cryogel and Hydrogel Synthesis with Hydroxyapatite Doping

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HEMA, piperazine (PIPz, 99%), di(ethylene glycol)diacrylate and gelatin (type A, porcine), potassium persulfate (PPS, anhydrous), N,N,N′,N′-tetraethylethylene diamine (TEMED), N-Ethyl-N′-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC), sodium hydrogen carbonate (NaHCO3), and polyethylene glycol sorbitan monolaurate (TWEEN 20) used in the cryogel and hydrogel synthesis were purchased from Sigma-Aldrich (St. Louis, MI, USA). All cryogel and hydrogel syntheses were performed in deionized water. Aqueous buffer solutions were prepared using potassium hydrogen phosphates (KH2PO4 and K2HPO4, Sigma-Aldrich) and deionized water. Materials used for the hydroxyapatite synthesis and doping were calcium nitrate pentahydrate (Ca(NO3)2 × 5H2O) (Sigma-Aldrich), magnesium nitrate hexahydrate (Mg(NO3)2 × 6H2O), strontium nitrate (Sr(NO3)2), gallium nitrate hydrate (Ga(NO3)3 × H2O), zinc nitrate hexahydrate (Zn(NO3)2 × 6H2O), and ammonium dihydrogen phosphate (NH4H2PO4) were obtained from Sigma-Aldrich, and urea ((NH2)2CO) was purchased from Alfa Aesar (Haverhill, MA, USA), and all were of analytical grade. All experiments were performed using lab-produced, ultra-distilled water.
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