Ab22450

Manufactured by Abcam

Ab22450 is a laboratory equipment product offered by Abcam. It serves as a tool for conducting various scientific experiments and analyses. The core function of this product is to provide a specific capability required for laboratory research, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab36367, by Abcam (1 mentions) Avidin biotin peroxidase detection system, by Vector Laboratories (1 mentions) Ab144p, by Abcam (1 mentions) Prb 435p, by Merck Group (1 mentions) 3 3 diaminobenzidine dab substrate, by Merck Group (1 mentions) Mab377, by Fortrea (1 mentions)

1 protocol using ab22450

Immunofluorescence and Immunochemistry Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence and immunochemistry were performed as previously described (Cosset et al., 2015 (link), 2016 (link)). The following primary antibodies were used: mouse anti-neuronal nuclei-specific protein (NeuN) (Chemicon;MAB377), rabbit anti-βIII-tubulin (Covance;PRB435P), goat anti-ChaT (Chemicon; AB144P), rabbit anti-HB-9 (Abcam; ab922606), rabbit anti-ISLET1 (Abcam; ab22450), goat anti-GALR3 (Abcam), mouse anti-EV-71 (Abcam; ab36367), and mouse anti-PV-3 (Abcam; ab22450). Alexa Fluor (555 and 488)-labeled antibodies from goat or donkey against mouse, goat, or rabbit (Molecular Probes) were used as secondary antibodies. Cell nuclei were stained with DAPI (4, 6-diamidino-2-phenylindole). For IHC, biotin-conjugated anti-rabbit IgG or anti-goat IgG were used and developed using avidin-biotin peroxidase detection system (Vector Labs) with 3,3′-diaminobenzidine substrate (DAB, Sigma-Aldrich) after 2 min of incubation.

Cosset É., Hibaoui Y., Ilmjärv S., Dietrich P.Y., Tapparel C, & Krause K.H. (2021). Modeling Poliovirus Infection Using Human Engineered Neural Tissue Enriched With Motor Neuron Derived From Embryonic Stem Cells. Frontiers in Cell and Developmental Biology, 8, 593106.

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