Log In Sign up
The largest database of trusted experimental protocols

Click it edu alexa flour 594 kit

Manufactured by Beyotime
Visit Supplier

The Click-iT EdU Alexa Fluor 594 kit is a reagent kit used for the detection and quantification of cellular proliferation. It incorporates the nucleoside analog EdU (5-ethynyl-2'-deoxyuridine) which is incorporated into newly synthesized DNA during the S-phase of the cell cycle. The incorporated EdU is then detected using a copper-catalyzed click reaction with the Alexa Fluor 594 dye, allowing for fluorescent labeling and visualization of proliferating cells.

Automatically generated - may contain errors

Lab products found in correlation

488 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions) Ab6326, by Abcam (2 mentions)

Spelling variants of this product

EdU kits (2 citations) Alexa Flour 594 (1 citations)

2 protocols using click it edu alexa flour 594 kit

1

Proliferation Quantification in Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five-micrometer tissue paraffin sections were dewaxed, hydrated, incubated in 1 M hydrochloric acid at 37°C for twenty minutes, washed with PBS for three times and antigen retrieval was performed in 10 mM citric acid. The sections were then stained according to the manufacturer’s instructions using the Click-iT EdU Alexa Flour 594 kit (Beyotime, C0078S). After staining, the sections were incubated with blocking solution (Beyotime, P0102) for one hour at room temperature and incubated with primary antibody against BrdU (Abcam, ab6326,1:100) overnight at 37°C. The sections were washed for three times, and incubated with 488-conjugated secondary antibodies (Thermo Fisher, A11006,1:400) for one hour at room temperature, stained with DAPI for eight minutes, and finally mounted with anti-fluorescence quenching sealing medium.
Sheng X., Lin Z., Lv C., Shao C., Bi X., Deng M., Xu J., Guerrero-Juarez C.F., Li M., Wu X., Zhao R., Yang X., Li G., Liu X., Wang Q., Nie Q., Cui W., Gao S., Zhang H., Liu Z., Cong Y., Plikus M.V., Lengner C.J., Andersen B., Ren F, & Yu Z. (2020). Cycling Stem Cells Are Radioresistant and Regenerate the Intestine. Cell reports, 32(4), 107952.
+ Open protocol
+ Expand
2

Proliferation Quantification in Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five-micrometer tissue paraffin sections were dewaxed, hydrated, incubated in 1 M hydrochloric acid at 37°C for twenty minutes, washed with PBS for three times and antigen retrieval was performed in 10 mM citric acid. The sections were then stained according to the manufacturer’s instructions using the Click-iT EdU Alexa Flour 594 kit (Beyotime, C0078S). After staining, the sections were incubated with blocking solution (Beyotime, P0102) for one hour at room temperature and incubated with primary antibody against BrdU (Abcam, ab6326,1:100) overnight at 37°C. The sections were washed for three times, and incubated with 488-conjugated secondary antibodies (Thermo Fisher, A11006,1:400) for one hour at room temperature, stained with DAPI for eight minutes, and finally mounted with anti-fluorescence quenching sealing medium.
Sheng X., Lin Z., Lv C., Shao C., Bi X., Deng M., Xu J., Guerrero-Juarez C.F., Li M., Wu X., Zhao R., Yang X., Li G., Liu X., Wang Q., Nie Q., Cui W., Gao S., Zhang H., Liu Z., Cong Y., Plikus M.V., Lengner C.J., Andersen B., Ren F, & Yu Z. (2020). Cycling Stem Cells Are Radioresistant and Regenerate the Intestine. Cell reports, 32(4), 107952.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!